ArticleNucleic acids research1994
Developmentally regulated, low abundance Tec element transcripts in Euplotes crassus--implications for DNA elimination and transposition.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Evolutionary origins and impacts of genome architecture in ciliates.Annals of the New York Academy of Sciences · 2019Review
- Flow cytometry sorting of nuclei enables the first global characterization of Paramecium germline DNA and transposable elements.BMC genomics · 2017Article
- Processing of double-strand breaks is involved in the precise excision of paramecium internal eliminated sequences.Molecular and cellular biology · 2003Article
- Selection on the genes of Euplotes crassus Tec1 and Tec2 transposons: evolutionary appearance of a programmed frameshift in a Tec2 gene encoding a tyrosine family site-specific recombinase.Eukaryotic cell · 2003Article
- Tec3, a new developmentally eliminated DNA element in Euplotes crassus.Eukaryotic cell · 2003Article
- Nongenic, bidirectional transcription precedes and may promote developmental DNA deletion in Tetrahymena thermophila.Genes & development · 2001Article
- A family of developmentally excised DNA elements in Tetrahymena is under selective pressure to maintain an open reading frame encoding an integrase-like protein.Nucleic acids research · 2000Article
- A nuclear protein involved in apoptotic-like DNA degradation in Stylonychia: implications for similar mechanisms in differentiating and starved cells.Molecular biology of the cell · 1999Article
- Article
- An unusual histone H3 specific for early macronuclear development in Euplotes crassus.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- A nascent micronuclear pseudogene in the ciliate Euplotes crassus.Nucleic acids research · 1996Article
- DNA rearrangements in Euplotes crassus coincide with discrete periods of DNA replication during the polytene chromosome stage of macronuclear development.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During macromolecular development in the ciliated protozoan, Euplotes crassus, > 105 Tec elements are precisely eliminated from the genome in a 2-4 h time interval, generating extrachromosomal circular forms of the elements. Various models have proposed a transposition-based mechanism for this excision. We have tested this hypothesis by determining the abundance of transcripts of Tec element open reading frames (ORFs) and the timing of their appearance. Transcripts are very low in abundance and are only detected by PCR amplification techniques. Thus, the low levels of transcripts argue against the participation of element-encoded functions in the Tec element elimination process. The element transcripts are only detected in RNA samples from mated cells, indicating that the micronucleus and/or developing macronucleus are transcriptionally active during the sexual phase of the life cycle. The transcription detected could allow a low level of germline-specific transposition for these elements.
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