ArticleNucleic acids research1994
SARs stimulate but do not confer position independent gene expression.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 90 citations in OpenAlex.
- Review
- Differential nuclear scaffold/matrix attachment marks expressed genes.Human molecular genetics · 2009Article
- Human matrix attachment regions are necessary for the establishment but not the maintenance of transgene insulation in Drosophila melanogaster.Molecular and cellular biology · 2004Article
- High efficiency, site-specific excision of a marker gene by the phage P1 cre-loxP system in the yellow fever mosquito, Aedes aegypti.Nucleic acids research · 2003Article
- Visualization of chromatin domains created by the gypsy insulator of Drosophila.The Journal of cell biology · 2003Article
- Assembly of two transgenes in an artificial chromatin domain gives highly coordinated expression in tobacco.Genetics · 2002Article
- The human growth hormone locus control region mediates long-distance transcriptional activation independent of nuclear matrix attachment regions.Nucleic acids research · 2001Article
- Use of matrix attachment regions (MARs) to minimize transgene silencing.Plant molecular biology · 2000Review
- Position-independent expression of transgenes in zebrafish.Transgenic research · 1999Article
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- Scaffold attachment region-mediated enhancement of retroviral vector expression in primary T cells.Journal of virology · 1998Article
- In vivo analysis of scaffold-associated regions in Drosophila: a synthetic high-affinity SAR binding protein suppresses position effect variegation.The EMBO journal · 1998Article
- Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk.Nature biotechnology · 1998Article
- Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster.Molecular and cellular biology · 1998Article
- The scs' boundary element: characterization of boundary element-associated factors.Molecular and cellular biology · 1997Article
- The heat shock cognate 80 gene of tomato is flanked by matrix attachment regions.Plant molecular biology · 1996Article
- Apoptosis and APC in colorectal tumorigenesis.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco.The Plant cell · 1996Article
- The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.Molecular & general genetics : MGG · 1996Article
- Contrasting effects of the SATB1 core nuclear matrix attachment region and flanking sequences of the keratin 18 gene in transgenic mice.Molecular biology of the cell · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two minimal scaffold-associated regions (SARs) from Drosophila were tested in stably transformed cells for their effects on the expression of reporter genes. The expression of genes bounded by two SARs is consistently stimulated by about 20- to 40-fold, if the average of a pool of cell transformants is analyzed. However, analysis of individual, stable cell transformants demonstrates that flanking SAR elements do not confer position-independent expression on the reporter gene and that the extent of position-dependent variegation is similarly large with or without the flanking SAR elements. The SAR stimulation of expression is observed in stable but not in transiently transfected cell lines. The Drosophila scs and scs' boundary elements, which do not bind to the nuclear matrix in vitro, are only about one-tenth as active as SARs in stimulating expression in stable transformants. Interestingly, the SAR stimulatory effect can be blocked by a fragment containing CpG islands (approximately 70% GC), if positioned between the SAR and the enhancer. In contrast, when inserted in the same position, control fragments, such as the scs/scs' elements, do not interfere with SAR function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.