ArticlePflugers Archiv : European journal of physiology1994
pH regulation in HT29 colon carcinoma cells.
Article in Pflugers Archiv : European journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 25 citations in OpenAlex.
- The electroneutral NaAmerican journal of physiology. Cell physiology · 2024Article
- Calcium dynamics during physiological acidification in Xenopus oocyte.The Journal of membrane biology · 2010Article
- Action of ANG II and ANP on colon epithelial cells.Pflugers Archiv : European journal of physiology · 2005Article
- The membrane transporters regulating epithelial NaCl secretion.Pflugers Archiv : European journal of physiology · 1996Review
- Effect of alkalinization of cytosolic pH by amines on intracellular Ca2+ activity in HT29 cells.Pflugers Archiv : European journal of physiology · 1996Article
- Whole-cell conductive properties of rat pancreatic acini.Pflugers Archiv : European journal of physiology · 1996Article
- 8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate (TMB-8) acts as a muscarinic receptor antagonist in the epithelial cell line HT29.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- N-Acetyl-L-cysteine and its derivatives activate a Cl- conductance in epithelial cells.Pflugers Archiv : European journal of physiology · 1996Article
- pH dependence of K+ conductances of rat cortical collecting duct principal cells.Pflugers Archiv : European journal of physiology · 1994Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The pH regulation in HT29 colon carcinoma cells has been investigated using the pH-sensitive fluorescent indicator 2',7'-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF). Under control conditions, intracellular pH (pHi) was 7.21 +/- 0.07 (n = 22) in HCO3(-)-containing and 7.21 +/- 0.09 (n = 12) in HCO3(-)-free solution. HOE-694 (10 mumol/l), a potent inhibitor of the Na+/H+ exchanger, did not affect control pHi. As a means to acidify cells we used the NH4+/NH3 (20 mmol/l) prepulse technique. The mean peak acidification was 0.37 +/- 0.07 pH units (n = 6). In HCO3(-)-free solutions recovery from acid load was completely blocked by HOE-694 (1 mumol/l), whereas in HCO3(-)-containing solutions a combination of HOE-694 and 4,4'-diisothiocyanatostilbene-2,2'-disulphonate (DIDS, 0.5 mmol/l) was necessary to show the same effect. Recovery from acid load was Na(+)-dependent in HCO3(-)-containing and HCO3(-)-free solutions. Removal of external Cl- caused a rapid, DIDS-blockable alkalinization of 0.33 +/- 0.03 pH units (n = 15) and of 0.20 +/- 0.006 pH units (n = 5), when external Na+ was removed together with Cl-. This alkalinization was faster in HCO3(-)-containing than in HCO3(-)-free solutions. The present observations demonstrate three distinct mechanisms of pHi regulation in HT29 cells: (a) a Na+/H+ exchanger, (b) a HCO3-/Cl- exchanger and (c) a Na(+)-dependent HCO3- transporter, probably the Na(+)-HCO3-/Cl- antiporter.(ABSTRACT TRUNCATED AT 250 WORDS)
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