ArticlePflugers Archiv : European journal of physiology1994
Oxytocin mobilizes calcium from a unique heparin-sensitive and thapsigargin-sensitive store in single myometrial cells from pregnant rats.
Article in Pflugers Archiv : European journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- Intradermal Injection of Oxytocin Aggravates Chloroquine-Induced Itch ResponsesFrontiers in pharmacology · 2019Article
- Oxytocin can regulate myometrial smooth muscle excitability by inhibiting the NaThe Journal of physiology · 2019Article
- Article
- Oxytocin-induced analgesia and scratching are mediated by the vasopressin-1A receptor in the mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Multiple signals regulate phospholipase CBeta3 in human myometrial cells.Biology of reproduction · 2008Article
- Hormonal signaling and signal pathway crosstalk in the control of myometrial calcium dynamics.Seminars in cell & developmental biology · 2007Review
- Inhibitory effect of alprostadil against sevoflurane-induced myometrial relaxation in rats.Journal of anesthesia · 2007Article
- Electrophysiological characterization and functional importance of calcium-activated chloride channel in rat uterine myocytes.Pflugers Archiv : European journal of physiology · 2004Article
- Identification and function of ryanodine receptor subtype 3 in non-pregnant mouse myometrial cells.The Journal of physiology · 2002Article
- Contribution of sarcoplasmic reticular calcium to smooth muscle contractile activation: gestational dependence in isolated rat uterus.The Journal of physiology · 1998Article
- Phospholipase C-delta1 and oxytocin receptor signalling: evidence of its role as an effector.The Biochemical journal · 1998Article
- Effect of estrogen upon cyclic ADP ribose metabolism: beta-estradiol stimulates ADP ribosyl cyclase in rat uterus.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Oxytocin receptor couples to the 80 kDa Gh alpha family protein in human myometrium.The Biochemical journal · 1996Article
- Identification of three isoforms of the InsP3 receptor in human myometrial smooth muscle.Pflugers Archiv : European journal of physiology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular free Ca2+ concentration ([Ca2+]i) was monitored using the fluorescence from the dye Fura-2-AM in single myometrial cells from pregnant rats. Oxytocin and acetylcholine applied to the cell evoked an initial peak in [Ca2+]i followed by a smaller sustained rise which was rapidly terminated upon removal of acetylcholine or persisted after oxytocin removal. A Ca2+ channel blocker (oxodipine) and external Ca2+ removal decreased both the transient and sustained rises in [Ca2+]i suggesting that Ca2+ influx through L-type Ca2+ channels participated in the global Ca2+ response induced by oxytocin. However, the initial peak in [Ca2+]i produced by oxytocin was mainly due to Ca2+ store release: it was abolished by inclusion of heparin [which blocks inositol 1,4,5-trisphosphate (InsP3) receptors] in the pipette (whole-cell recording mode of patch-clamp) and external application of thapsigargin (which blocks sarcoplasmic reticulum Ca(2+)-ATPases). In contrast, the transient Ca2+ response induced by oxytocin was unaffected by ryanodine. Moreover, caffeine failed to induce a rise in [Ca2+]i but reduced the oxytocin-induced transient Ca2+ response. The later sustained rise in [Ca2+]i produced by oxytocin was due to the entry of Ca2+ into the cell as it was suppressed in external Ca(2+)-free solution. The Ca2+ entry pathway is permeable to Mn2+ ions, in contrast to that described in various vascular and visceral smooth muscle cells. Oxytocin-induced Ca2+ release is blocked by the oxytocin antagonist d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH2(9)]OVT. The prolonged increase in [Ca2+]i after oxytocin removal is rapidly terminated by addition of the oxytocin antagonist suggesting that oxytocin dissociation from its receptor is very slow.(ABSTRACT TRUNCATED AT 250 WORDS)
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.