ArticleMolecular and cellular biology1994
Cellular aggregation enhances MyoD-directed skeletal myogenesis in embryonal carcinoma cells.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation.iScience · 2021Article
- Shaping Gene Expression by Landscaping Chromatin Architecture: Lessons from a Master.Molecular cell · 2018Review
- Myogenic progenitor specification from pluripotent stem cells.Seminars in cell & developmental biology · 2017Review
- Tumor Suppressors and Endodermal Differentiation of P19 Embryonic Stem Cells.Cell & developmental biology · 2015Article
- Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage.Cell reports · 2015Article
- Stimulated myoblast differentiation on graphene oxide-impregnated PLGA-collagen hybrid fibre matrices.Journal of nanobiotechnology · 2015Article
- Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices.Journal of biological engineering · 2015Article
- Skeletal muscle programming and re-programming.Current opinion in genetics & development · 2013Review
- Directed differentiation of size-controlled embryoid bodies towards endothelial and cardiac lineages in RGD-modified poly(ethylene glycol) hydrogels.Advanced healthcare materials · 2013Article
- Genetic and epigenetic determinants of neurogenesis and myogenesis.Developmental cell · 2012Article
- Gli2 and MEF2C activate each other's expression and function synergistically during cardiomyogenesis in vitro.Nucleic acids research · 2012Article
- Menin induces endodermal differentiation in aggregated P19 stem cells by modulating the retinoic acid receptors.Molecular and cellular biochemistry · 2012Article
- MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.The Journal of biological chemistry · 2011Article
- Chemical induction of cardiac differentiation in p19 embryonal carcinoma stem cells.Stem cells and development · 2010Article
- Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.Cell stem cell · 2009Article
- Cdc42-mTOR signaling pathway controls Hes5 and Pax6 expression in retinoic acid-dependent neural differentiation.The Journal of biological chemistry · 2009Article
- Beta-catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Netrins and neogenin promote myotube formation.The Journal of cell biology · 2004Article
- Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The E2 ubiquitin conjugase Rad6 is required for the ArgR/Mcm1 repression of ARG1 transcription.Molecular and cellular biology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When introduced into P19 embryonal carcinoma cells, recombinant genes encoding MyoD converted only a small percentage (< 3%) of the transfected cells into skeletal muscle. We isolated stably transfected cells that expressed the MyoD transcript. These P19[MyoD] cells continued to express markers characteristic of undifferentiated stem cells but also expressed myf-5 and the myotonic dystrophy kinase, transcripts normally present in myoblasts but absent from P19 cells. Aggregation of P19[MyoD] cells induced the expression of myogenin, desmin, and the retinoblastoma protein and resulted in the rapid and abundant development of skeletal muscle. Both the embryonic and the slow isoforms of myosin heavy chain were present in this muscle, indicating that it resembled skeletal muscle formed from primary myoblasts. Since aggregation of P19 cells normally results in inefficient differentiation and the development of only low levels of cardiac muscle but no skeletal muscle, we conclude that MyoD imposes the skeletal muscle program on P19 cells and that the differentiation of these cells requires inductive events provided by cell aggregation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.