ArticleMolecular and cellular biology1994
Transposition of the yeast retroviruslike element Ty3 is dependent on the cell cycle.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Diverse transposable element landscapes in pathogenic and nonpathogenic yeast models: the value of a comparative perspective.Mobile DNA · 2020Review
- Structure of the Ty3/Gypsy retrotransposon capsid and the evolution of retroviruses.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3.Viruses · 2016Review
- Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes.PLoS genetics · 2015Article
- Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation.G3 (Bethesda, Md.) · 2011Article
- The TY3 Gag3 spacer controls intracellular condensation and uncoating.Journal of virology · 2011Article
- Two-hybrid analysis of Ty3 capsid subdomain interactions.Mobile DNA · 2010Article
- Ty3 nuclear entry is initiated by viruslike particle docking on GLFG nucleoporins.Journal of virology · 2009Article
- Ty3 nucleocapsid controls localization of particle assembly.Journal of virology · 2008Article
- Ty3 capsid mutations reveal early and late functions of the amino-terminal domain.Journal of virology · 2007Article
- Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components.RNA (New York, N.Y.) · 2006Article
- Investigation by atomic force microscopy of the structure of Ty3 retrotransposon particles.Journal of virology · 2005Article
- Retroviruses and yeast retrotransposons use overlapping sets of host genes.Genome research · 2005Article
- Functional roles of carboxylate residues comprising the DNA polymerase active site triad of Ty3 reverse transcriptase.Nucleic acids research · 2005Article
- Article
- Ty3 integrase is required for initiation of reverse transcription.Journal of virology · 2002Article
- A truncation mutant of the 95-kilodalton subunit of transcription factor IIIC reveals asymmetry in Ty3 integration.Molecular and cellular biology · 2001Article
- Integrase mediates nuclear localization of Ty3.Molecular and cellular biology · 2001Article
- Ten-kilodalton domain in Ty3 Gag3-Pol3p between PR and RT is dispensable for Ty3 transposition.Journal of virology · 2001Article
- Yeast heterochromatin is a dynamic structure that requires silencers continuously.Genes & development · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Host cell cycle genes provide important functions to retroviruses and retroviruslike elements. To define some of these functions, the cell cycle dependence of transposition of the yeast retroviruslike element Ty3 was examined. Ty3 is unique among retroviruslike elements because of the specificity of its integration, which occurs upstream of genes transcribed by RNA polymerase III. A physical assay for Ty3 transposition which takes advantage of this position-specific integration was developed. The assay uses PCR to amplify a product of Ty3 integration into a target plasmid that carries a modified tRNA gene. By using the GAL1 upstream activating sequence to regulate expression of Ty3, transposition was detected within one generation of cell growth after Ty3 transcription was initiated. This physical assay was used to show that Ty3 did not transpose when yeast cells were arrested in G1 during treatment with the mating pheromone alpha-factor. The restriction of transposition was not due to changes in transcription of either Ty3 or tRNA genes or to aspects of the mating pheromone response unrelated to cell cycle arrest. The block of the Ty3 life cycle was reversed when cells were released from G1 arrest. Examination of Ty3 intermediates during G1 arrest indicated that Ty3 viruslike particles were present but that reverse transcription of the Ty3 genomic RNA into double-stranded DNA had not occurred. In G1, the Ty3 life cycle is blocked after particle assembly but before the completion of reverse transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.