ArticleThe Journal of membrane biology1994
Apical membrane Cl-butyrate exchange: mechanism of short chain fatty acid stimulation of active chloride absorption in rat distal colon.
Article in The Journal of membrane biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 64 citations in OpenAlex.
- Unlocking gut-liver-brain axis communication metabolites: energy metabolism, immunity and barriers.NPJ biofilms and microbiomes · 2024Review
- Involvement of butyrate in electrogenic KPflugers Archiv : European journal of physiology · 2019Article
- SLC9 Gene Family: Function, Expression, and Regulation.Comprehensive Physiology · 2018Review
- Short- and medium-chain fatty acids in energy metabolism: the cellular perspective.Journal of lipid research · 2016Review
- Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na+ Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis.The Journal of biological chemistry · 2015Article
- Oral rehydration therapy in the second decade of the twenty-first century.Current gastroenterology reports · 2014Review
- Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells.Pflugers Archiv : European journal of physiology · 2007Article
- Transport of acetate and sodium in sheep omasum: mutual, but asymmetric interactions.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2006Article
- Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon.Digestive diseases and sciences · 2004Article
- Acute regulation of the SLC26A3 congenital chloride diarrhoea anion exchanger (DRA) expressed in Xenopus oocytes.The Journal of physiology · 2003Article
- Effects of butyrate on active sodium and chloride transport in rat and rabbit distal colon.The Journal of physiology · 2002Article
- Stimulation of sodium chloride absorption from secreting rat colon by short-chain fatty acids.Digestive diseases and sciences · 1999Article
- Maintenance and regulation of the pH microclimate at the luminal surface of the distal colon of guinea-pig.The Journal of physiology · 1999Article
- The glow of the colonic pH microclimate kindled by short-chain fatty acids, chloride and bicarbonate.The Journal of physiology · 1999Review
- Non-ionic diffusion and carrier-mediated transport drive extracellullar pH regulation of mouse colonic crypts.The Journal of physiology · 1996Article
- An Na(+)-independent short-chain fatty acid transporter contributes to intracellular pH regulation in murine colonocytes.The Journal of general physiology · 1995Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The cellular model of short chain fatty acid stimulation of electroneutral Na-Cl absorption in large intestine proposes that SCFA, following its uptake across the apical membrane, recycles and is coupled to functional Na-H and Cl-short chain fatty acid exchanges. To establish the presence of a Cl-butyrate exchange (used as a model short chain fatty acid), studies of 36Cl and 14C-butyrate uptake across apical membrane vesicles of rat distal colon were performed. An outward butyrate-gradient stimulated transient accumulation of 36Cl uptake that was not inhibited by pH clamping with valinomycin (a K ionophore) and FCCP (a proton ionophore). Outward butyrate-gradient-stimulated 36Cl uptake was inhibited by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) with a half-maximal inhibitory concentration (IC50) of 68.4 microM, and was saturated by both increasing extravesicular Cl concentration (Km for Cl of 26.8 +/- 3.4 mM and a Vmax of 12.4 +/- 0.6 nmol/mg protein x 9 sec) and increasing intravesicular butyrate concentration (Km for butyrate of 5.9 mM and a Vmax for Cl of 5.9 nmol/mg protein x 9 sec). 36Cl uptake was also stimulated by outward gradients of other short chain fatty acids (e.g., propionate, acetate and formate). In contrast, an outward Cl gradient failed to enhance 14C-butyrate uptake. Extravesicular Cl more than extravesicular butyrate enhanced 36Cl efflux from apical membrane vesicles. These studies provide compelling evidence for the presence of an electroneutral, pH-activated, Cl-butyrate exchange which in concert with Na-H exchange is the mechanism by which butyrate stimulates electroneutral Na-Cl absorption.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.