ArticleThe Journal of cell biology1994
Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Spatial variation of microtubule depolymerization in large asters.Molecular biology of the cell · 2021Article
- Calcium signaling and meiotic exit at fertilization in Xenopus egg.International journal of molecular sciences · 2014Review
- The elegans of spindle assembly.Cellular and molecular life sciences : CMLS · 2010Review
- Interactions between EB1 and microtubules: dramatic effect of affinity tags and evidence for cooperative behavior.The Journal of biological chemistry · 2009Article
- Cdc2-mediated phosphorylation of Kid controls its distribution to spindle and chromosomes.The EMBO journal · 2003Article
- XMAP215 regulates microtubule dynamics through two distinct domains.The EMBO journal · 2001Article
- TPX2, A novel xenopus MAP involved in spindle pole organization.The Journal of cell biology · 2000Article
- Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster.The Journal of cell biology · 2000Article
- D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo.The EMBO journal · 2000Article
- mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.The Journal of cell biology · 1999Article
- Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis.Molecular biology of the cell · 1999Article
- ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.The Journal of cell biology · 1998Article
- XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle.The Journal of cell biology · 1997Article
- The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle.The Journal of cell biology · 1997Article
- Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level.The Journal of cell biology · 1996Article
- Domains of tau protein, differential phosphorylation, and dynamic instability of microtubules.Molecular biology of the cell · 1995Article
- Binding of E-MAP-115 to microtubules is regulated by cell cycle-dependent phosphorylation.The Journal of cell biology · 1995Article
- Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras.The Journal of cell biology · 1995Article
- Cyclins and cyclin-dependent kinases: a biochemical view.The Biochemical journal · 1995Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reorganization from a radial [corrected] interphase microtubule (MT) network into a bipolar spindle at the onset of mitosis involves a dramatic change in MT dynamics. Microtubule-associated proteins (MAPs) and other factors are thought to regulate MT dynamics both in interphase and in mitosis. In this study we report the purification and functional in vitro characterization of a 230-KD MAP from Xenopus egg extract (XMAP230). This protein is present in eggs, oocytes, testis and a Xenopus tissue culture cell line. It is apparently absent from non-dividing cells in which an immunologically related 200-kD protein is found. XMAP230 is composed of two isoforms with slightly different molecular masses and pIs. It is localized to interphase MTs, dissociates from MTs at the onset of prophase and specifically binds to spindle MTs during metaphase and anaphase. The dissociation constant of XMAP230 is 500 nM, the stoichiometry of binding to MTs is between 1:8 and 1:4, and the in vivo concentration is approximately 200 nM. Both isoforms are phosphorylated and have reduced affinity for microtubules in mitotic extracts. Analysis of the effect of XMAP230 on MT dynamics by video microscopy shows that it increases the growth rate, decreases the shrinking rate of MTs and strongly suppresses catastrophes. These results suggest that in vivo, XMAP230 participates in the control of the MT elongation rate, stabilizes MTs and locally modulates MT dynamics during mitosis.
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