ArticleThe Journal of cell biology1994
Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 97 citations in OpenAlex.
- Direct observation of interdependent and hierarchical kinetochore assembly on individual centromeres.Nucleic acids research · 2025Article
- Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification.Life science alliance · 2025Article
- Kinetochores grip microtubules with directionally asymmetric strength.The Journal of cell biology · 2025Article
- De novo assembly and single-molecule study of kinetochore-microtubule interactions.Frontiers in cell and developmental biology · 2025Article
- Concatemer Assisted Stoichiometry Analysis (CASA): targeted mass spectrometry for protein quantification.bioRxiv : the preprint server for biology · 2024Article
- Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.Molecular biology of the cell · 2021Article
- Identification and characterization of centromeric sequences inGenome research · 2021Article
- An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.eLife · 2018Article
- In Vitro Kinetochore Assembly.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Review
- A cell-free system for functional centromere and kinetochore assembly.Nature protocols · 2012Article
- Review
- Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation.Molecular biology of the cell · 2011Article
- Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp.Methods (San Diego, Calif.) · 2010Review
- The life and miracles of kinetochores.The EMBO journal · 2009Review
- Cohesin interaction with centromeric minichromosomes shows a multi-complex rod-shaped structure.PloS one · 2008Article
- Kinetochore structure and spindle assembly checkpoint signaling in the budding yeast, Saccharomyces cerevisiae.Frontiers in bioscience : a journal and virtual library · 2008Review
- Insights into kinetochore-DNA interactions from the structure of Cep3Delta.EMBO reports · 2008Article
- Article
- Suppressor analysis of a histone defect identifies a new function for the hda1 complex in chromosome segregation.Genetics · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetochores are structures that assemble on centromeric DNA and mediate the attachment of chromosomes to the microtubules of the mitotic spindle. The protein components of kinetochores are poorly understood, but the simplicity of the S. cerevisiae kinetochore makes it an attractive candidate for molecular dissection. Mutations in genes encoding CBF1 and CBF3, proteins that bind to yeast centromeres, interfere with chromosome segregation in vivo. To determine the roles played by these factors and by various regions of centromeric DNA in kinetochore function, we have developed a method to partially reassemble kinetochores on exogenous centromeric templates in vitro and to visualize the attachment of these reassembled kinetochore complexes to microtubules. In this assay, single reassembled complexes appear to mediate microtubule binding. We find that CBF3 is absolutely essential for this attachment but, contrary to previous reports (Hyman, A. A., K. Middleton, M. Centola, T.J. Mitchison, and J. Carbon. 1992. Microtubule-motor activity of a yeast centromere-binding protein complex. Nature (Lond.). 359:533-536) is not sufficient. Additional cellular factors interact with CBF3 to form active microtubule-binding complexes. This is mediated primarily by the CDEIII region of centromeric DNA but CDEII plays an essential modulatory role. Thus, the attachment of kinetochores to microtubules appears to involve a hierarchy of interactions by factors that assemble on a core complex consisting of DNA-bound CBF3.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.