ArticleThe Journal of cell biology1994
Vinculin-deficient PC12 cell lines extend unstable lamellipodia and filopodia and have a reduced rate of neurite outgrowth.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 90 citations in OpenAlex.
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- Förster resonance energy transfer efficiency of the vinculin tension sensor in cultured primary cortical neuronal growth cones.Neurophotonics · 2022Article
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- Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons.Cellular and molecular life sciences : CMLS · 2021Article
- Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.Frontiers in bioengineering and biotechnology · 2018Article
- Alpha 7 nicotinic receptors attenuate neurite development through calcium activation of calpain at the growth cone.PloS one · 2018Article
- Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation.Scientific reports · 2017Article
- Vinculin forms a directionally asymmetric catch bond with F-actin.Science (New York, N.Y.) · 2017Article
- Molecular events in the cell types of the olfactory epithelium during adult neurogenesis.Molecular brain · 2013Article
- Alignment and composition of laminin-polycaprolactone nanofiber blends enhance peripheral nerve regeneration.Journal of biomedical materials research. Part A · 2012Article
- Homocysteine induces hypophosphorylation of intermediate filaments and reorganization of actin cytoskeleton in C6 glioma cells.Cellular and molecular neurobiology · 2010Article
- Modulation of cell spreading and cell-substrate adhesion dynamics by dystroglycan.Journal of cell science · 2010Article
- Retinotopic mapping requires focal adhesion kinase-mediated regulation of growth cone adhesion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Molecular noise of capping protein binding induces macroscopic instability in filopodial dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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- Axon extension in the fast and slow lanes: substratum-dependent engagement of myosin II functions.Developmental neurobiology · 2007Article
- Studies on integrins in the nervous system.Methods in enzymology · 2007Review
- Rac1 and RhoA promote neurite outgrowth through formation and stabilization of growth cone point contacts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- ARNO and ARF6 regulate axonal elongation and branching through downstream activation of phosphatidylinositol 4-phosphate 5-kinase alpha.Molecular biology of the cell · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the role of vinculin in regulating integrin-dependent neurite outgrowth in PC12 cells, a neuronal cell line. Vinculin is a cytoskeletal protein believed to mediate interactions between integrins and the actin cytoskeleton. In differentiated PC12 cells, the cell body, the neurite, and the growth cone contain vinculin. Within the growth cone, both the proximal region of "consolidation" and the more distal region consisting of lamellipodia and filopodia contain vinculin. To study the role of vinculin in neurite outgrowth, we generated vinculin-deficient isolates of PC12 cell lines by transfection with vectors expressing antisense vinculin RNA. In some of these cell lines, vinculin levels were reduced to 18-23% of normal levels. In the vinculin-deficient cell lines, neurite outgrowth on laminin was significantly reduced. In time-lapse analysis, growth cones advanced much more slowly than normal. Further analysis indicated that this deficit could be explained in large part by changes in the behaviors of filopodia and lamellipodia. Filopodia were formed in normal numbers, extended at normal rates, and extended to approximately normal lengths, but were much less stable in the vinculin deficient compared to control PC12 cells. Similarly, lamellipodia formed and grew nearly normally, but were dramatically less stable in the vinculin-deficient cells. This can account for the reduction in rate of growth cone advance. These results indicate that interactions between integrins and the actin-based cytoskeleton are necessary for stability of both filopodia and lamellipodia.
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