ArticleThe Journal of cell biology1994
Transport via the regulated secretory pathway in semi-intact PC12 cells: role of intra-cisternal calcium and pH in the transport and sorting of secretogranin II.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Article
- Dinoflagellate Amphiesmal Dynamics: Cell Wall Deposition with Ecdysis and Cellular Growth.Marine drugs · 2023Review
- Organellar Calcium Handling in the Cellular Reticular Network.Cold Spring Harbor perspectives in biology · 2019Review
- Golgi pH, Ion and Redox Homeostasis: How Much Do They Really Matter?Frontiers in cell and developmental biology · 2019Article
- Yeast Gdt1 is a Golgi-localized calcium transporter required for stress-induced calcium signaling and protein glycosylation.Scientific reports · 2016Article
- Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.Experimental dermatology · 2012Article
- Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.Chemical communications (Cambridge, England) · 2012Article
- Intracellular organelles in the saga of Ca2+ homeostasis: different molecules for different purposes?Cellular and molecular life sciences : CMLS · 2012Review
- The trans-golgi compartment: A new distinct intracellular Ca store.Communicative & integrative biology · 2010Article
- Unique characteristics of Ca2+ homeostasis of the trans-Golgi compartment.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A membrane-proximal region in the C-terminal tail of NHE7 is required for its distribution in the trans-Golgi network, distinct from NHE6 localization at endosomes.The Journal of membrane biology · 2010Article
- Pro-hormone secretogranin II regulates dense core secretory granule biogenesis in catecholaminergic cells.The Journal of biological chemistry · 2010Article
- Proinsulin endoproteolysis confers enhanced targeting of processed insulin to the regulated secretory pathway.Molecular biology of the cell · 2000Article
- Homotypic fusion of immature secretory granules during maturation in a cell-free assay.The Journal of cell biology · 1998Article
- The Golgi apparatus is an inositol 1,4,5-trisphosphate-sensitive Ca2+ store, with functional properties distinct from those of the endoplasmic reticulum.The EMBO journal · 1998Article
- Biogenesis of secretory granules in the trans-Golgi network of neuroendocrine and endocrine cells.Biochimica et biophysica acta · 1998Review
- The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein.The Journal of cell biology · 1998Article
- The medial-Golgi ion pump Pmr1 supplies the yeast secretory pathway with Ca2+ and Mn2+ required for glycosylation, sorting, and endoplasmic reticulum-associated protein degradation.Molecular biology of the cell · 1998Article
- Interaction of furin in immature secretory granules from neuroendocrine cells with the AP-1 adaptor complex is modulated by casein kinase II phosphorylation.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To gain insight into the mechanisms governing protein sorting, we have developed a system that reconstitutes both the formation of immature secretory granules and their fusion with the plasma membrane. Semi-intact PC12 cells were incubated with ATP and cytosol for 15 min to allow immature granules to form, and then in a buffer containing 30 microM [Ca2+]free to induce exocytosis. Transport via the regulated pathway, as assayed by the release of secretogranin II (SgII) labeled in the TGN, was inhibited by depletion of ATP, or by the inclusion of 100 microM GTP gamma S, 50 microM AlF3-5 or 5 micrograms/ml BFA. When added after immature granules had formed, GTP gamma S stimulated rather than inhibited exocytosis. Thus, exocytosis of immature granules in this system resembles the characteristics of fully matured granules. Transport of SgII via the regulated pathway occurred at a fourfold higher efficiency than glycosaminoglycan chains, indicating that SgII is sorted to some extent upon exit from the TGN. Addition of A23187 to release Ca2+ from the TGN had no significant effect on sorting of SgII into immature granules. In contrast, depletion of lumenal calcium inhibited the endoproteolytic cleavage of POMC and proinsulin. These results establish the importance of intra-cisternal Ca2+ in prohormone processing, but raise the question whether lumenal calcium is required for proper sorting of SgII into immature granules. Disruption of organelle pH gradients with an ionophore or a weak base resulted in the inhibition of transport via both the constitutive and the regulated pathways.
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