ArticleJournal of bacteriology1994
Localization of DnaK (chaperone 70) from Escherichia coli in an osmotic-shock-sensitive compartment of the cytoplasm.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- A Periplasmic Complex of the Nitrite Reductase NirS, the Chaperone DnaK, and the Flagellum Protein FliC Is Essential for Flagellum Assembly and Motility in Pseudomonas aeruginosa.Journal of bacteriology · 2015Article
- Differential proteomics of Helicobacter pylori associated with autoimmune atrophic gastritis.Molecular medicine (Cambridge, Mass.) · 2014Article
- How does Tremblaya princeps get essential proteins from its nested partner Moranella endobia in the Mealybug Planoccocus citri?PloS one · 2013Article
- Functional characterization of a small heat shock protein from Mycobacterium leprae.BMC microbiology · 2008Article
- Molecular sieve mechanism of selective release of cytoplasmic proteins by osmotically shocked Escherichia coli.Journal of bacteriology · 2001Article
- Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin.Journal of bacteriology · 2000Article
- Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF.Journal of bacteriology · 2000Article
- Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscL.Journal of bacteriology · 2000Article
- Identification of a 71-kilodalton surface-associated Hsp70 homologue in Coxiella burnetii.Infection and immunity · 1998Article
- Leaderless polypeptides efficiently extracted from whole cells by osmotic shock.Journal of bacteriology · 1997Article
- Yersinia pestis LcrV forms a stable complex with LcrG and may have a secretion-related regulatory role in the low-Ca2+ response.Journal of bacteriology · 1997Article
- Elevated levels of Legionella pneumophila stress protein Hsp60 early in infection of human monocytes and L929 cells correlate with virulence.Infection and immunity · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chaperone DnaK can be released (up to 40%) by osmotic shock, a procedure which is known to release the periplasmic proteins and a select group of cytoplasmic proteins (including thioredoxin and elongation factor Tu) possibly associated with the inner face of the inner membrane. As distinct from periplasmic proteins, DnaK is retained within spheroplasts prepared with lysozyme and EDTA. The ability to isolate DnaK with a membrane fraction prepared under gentle lysis conditions supports a peripheral association between DnaK and the cytoplasmic membrane. Furthermore, heat shock transiently increases the localization of DnaK in the osmotic-shock-sensitive compartment of the cytoplasm. We conclude that DnaK belongs to the select group of cytoplasmic proteins released by osmotic shock, which are possibly located at Bayer adhesion sites, where the inner and outer membranes are contiguous.
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