ArticleJournal of bacteriology1994
Quantitative discrimination of carrier-mediated excretion of isoleucine from uptake and diffusion in Corynebacterium glutamicum.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Metabolic engineering strategies for sustainable de novo isobutylamine production by Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2026Article
- Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.Applied and environmental microbiology · 2015Article
- Production of 2-ketoisocaproate with Corynebacterium glutamicum strains devoid of plasmids and heterologous genes.Microbial biotechnology · 2015Article
- Effect of transport proteins on L-isoleucine production with the L-isoleucine-producing strain Corynebacterium glutamicum YILW.Journal of industrial microbiology & biotechnology · 2012Article
- Characterization of methionine export in Corynebacterium glutamicum.Journal of bacteriology · 2005Article
- Export of L-isoleucine from Corynebacterium glutamicum: a two-gene-encoded member of a new translocator family.Journal of bacteriology · 2002Article
- L-threonine export: use of peptides to identify a new translocator from Corynebacterium glutamicum.Journal of bacteriology · 2001Article
- l-Isoleucine Production with Corynebacterium glutamicum: Further Flux Increase and Limitation of Export.Applied and environmental microbiology · 1996Article
- Mechanism and Regulation of Isoleucine Excretion in Corynebacterium glutamicum.Applied and environmental microbiology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The efflux of isoleucine in whole cells of Corynebacterium glutamicum was studied. The different amino acid fluxes across the plasma membrane were functionally discriminated into passive diffusion, carrier-mediated excretion, and carrier-mediated uptake. Detailed kinetic analysis was made possible by controlled variation of internal isoleucine from low concentrations to 100 mM by feeding with mixtures of isoleucine-containing peptides. Isoleucine diffusion was experimentally separated and proceeded with a first-order rate constant of 0.083 min-1 or 0.13 microliters.min-1.mg (dry mass)-1, which corresponds to a permeability of 2 x 10(-8) cm.s-1. Uptake of isoleucine was constant at a rate of 1.1 nmol.min-1.mg (dry mass)-1. Carrier-mediated isoleucine excretion was zero below a threshold of 8 mM cytosolic isoleucine. Above this level, a Michaelis-Menten-type kinetics was observed, with a Km of 21 mM (13 mM plus 8 mM threshold value) and a Vmax of 14.5 nmol.min-1.mg (dry mass)-1. The activity of the isoleucine excretion carrier depended on the presence of a membrane potential. Excretion was specific for L-isoleucine (and presumably L-leucine) and could be inhibited by SH reagents.
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