ArticleJournal of anatomy1994
Membrane skeleton in fresh unfixed erythrocytes as revealed by a rapid-freezing and deep-etching method.
Article in Journal of anatomy, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Multimodal imaging reveals membrane skeleton reorganisation during reticulocyte maturation and differences in dimple and rim regions of mature erythrocytes.Journal of structural biology: X · 2022Article
- Native ultrastructure of the red cell cytoskeleton by cryo-electron tomography.Biophysical journal · 2011Article
- Morphological study by an 'in vivo cryotechnique' of the shape of erythrocytes circulating in large blood vessels.Journal of anatomy · 1998Article
- An immunocytochemical study of changes in the human erythrocyte membrane skeleton produced by stretching examined by the quick-freezing and deep-etching method.Journal of anatomy · 1997Article
- An elastic network model based on the structure of the red blood cell membrane skeleton.Biophysical journal · 1996Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid-freezing and deep-etching method for examining en face the cytoplasmic aspects of unfixed erythrocyte membranes is described, which provides improved resolution. Normal human erythrocytes were centrifuged, washed in a phosphate buffer solution and pelleted. Glass coverslips were coated with 3-aminopropyl triethoxy silane and glutaraldehyde to make erythrocytes stick to them. A drop containing the erythrocyte pellet was sandwiched between 2 coverslips. The attached erythrocytes were slowly split open in the cytosol buffer solution. The specimens on coverslips were rapidly frozen in an isopentane-propane mixture (-193 degrees C), deeply etched and rotary shadowed with platinum and carbon. Filamentous structures were observed to form fine networks on the cytoplasmic side of erythrocyte membranes. The length of the filaments was shorter than that previously reported for glutaraldehyde-fixed filaments. The number of intersections between filaments was increased as compared with the previous data. It is concluded that dense in situ networks of short filaments beneath erythrocyte membranes can be viewed in a relatively intact state by splitting fresh unfixed specimens followed by the rapid-freezing and deep-etching method.
Indexed as
Identifiers
7961147PMC1166771What OpenQuestion holds
Registered trials
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