ArticleThe EMBO journal1994
VSG gene expression site control in insect form Trypanosoma brucei.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 95 citations in OpenAlex.
- VEX1 Influences mVSG Expression During the Transition to Mammalian Infectivity inFrontiers in cell and developmental biology · 2022Article
- Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei.Molecular and biochemical parasitology · 2018Article
- Transcriptional Regulation of Telomeric Expression Sites and Antigenic Variation in Trypanosomes.Current genomics · 2018Review
- High-resolution analysis of multi-copy variant surface glycoprotein gene expression sites in African trypanosomes.BMC genomics · 2016Article
- Article
- Exposure of Trypanosoma brucei to an N-acetylglucosamine-binding lectin induces VSG switching and glycosylation defects resulting in reduced infectivity.PLoS neglected tropical diseases · 2015Article
- Identification of the glucosyltransferase that converts hydroxymethyluracil to base J in the trypanosomatid genome.The Journal of biological chemistry · 2014Article
- Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Role of expression site switching in the development of resistance to human Trypanosome Lytic Factor-1 in Trypanosoma brucei brucei.Molecular and biochemical parasitology · 2012Article
- Histone H1 plays a role in heterochromatin formation and VSG expression site silencing in Trypanosoma brucei.PLoS pathogens · 2012Article
- Endosomal localization of the serum resistance-associated protein in African trypanosomes confers human infectivity.Eukaryotic cell · 2011Article
- TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei.Eukaryotic cell · 2011Article
- NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei.Nucleic acids research · 2011Article
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- Mechanism of Trypanosoma brucei gambiense (group 1) resistance to human trypanosome lytic factor.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Active VSG expression sites in Trypanosoma brucei are depleted of nucleosomes.Eukaryotic cell · 2010Article
- A novel ISWI is involved in VSG expression site downregulation in African trypanosomes.The EMBO journal · 2007Article
- Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei.Nucleic acids research · 2004Article
- RNA polymerase I transcribes procyclin genes and variant surface glycoprotein gene expression sites in Trypanosoma brucei.Eukaryotic cell · 2003Article
- Ex vivo and in vitro identification of a consensus promoter for VSG genes expressed by metacyclic-stage trypanosomes in the tsetse fly.Eukaryotic cell · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When the African trypanosome Trypanosoma brucei is taken up from mammals by a tse-tse fly, it replaces its variant surface glycoprotein (VSG) coat by a procyclin coat. Transcription of VSG genes stops in the fly, but transcription of sequences derived from the promoter area of the VSG expression site(s) remains high. Whether this is due to continuing high activity of one promoter or to low activity of many promoters was unclear. We have used the small differences between the sequences of different expression sites to show that multiple expression site promoters are active in insect form trypanosomes. This is confirmed by the low expression of single copy marker genes introduced into the transcribed area. However, if the expression site promoter is removed from the genomic location of the expression site and inserted in the non-transcribed spacer of the ribosomal DNA (rDNA), it is derepressed. Derepression of transcription can also be accomplished by replacing the promoter of an expression site by an rDNA promoter. We conclude that the down-regulation of VSG gene expression site promoters in insect form trypanosomes is affected by both the DNA sequence of the promoter and the genomic context in which it resides.
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