ArticleThe EMBO journal1994
A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- TbENF is an essential TbTFIIB-interacting trypanosomatid-specific factor.Molecular and biochemical parasitology · 2012Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes.International journal for parasitology · 2010Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- Gene expression in trypanosomatid parasites.Journal of biomedicine & biotechnology · 2010Review
- The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes.BMC microbiology · 2007Article
- A novel class of developmentally regulated noncoding RNAs in Leishmania.Eukaryotic cell · 2006Article
- Complete cap 4 formation is not required for viability in Trypanosoma brucei.Eukaryotic cell · 2006Article
- Transcription initiation and termination on Leishmania major chromosome 3.Eukaryotic cell · 2004Article
- RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
- Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms.Nucleic acids research · 2001Article
- Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.Molecular and cellular biology · 1999Article
- Three small nucleolar RNAs identified from the spliced leader-associated RNA locus in kinetoplastid protozoans.Molecular and cellular biology · 1998Article
- Structure-function analysis of the trypanosomatid spliced leader RNA.Nucleic acids research · 1998Article
- Characterization of a novel trypanosomatid small nucleolar RNA.Nucleic acids research · 1998Article
- The U5 RNA of trypanosomes deviates from the canonical U5 RNA: the Leptomonas collosoma U5 RNA and its coding gene.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.The EMBO journal · 1996Article
- Control of gene expression in trypanosomes.Microbiological reviews · 1995Review
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3 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate that the mini-exon genes of Leishmania tarentolae are individually transcribed by an enzyme pharmacologically identified as RNA polymerase II. To study transcription in these ancient eukaryotes, a stable transformation assay using an episomal mini-exon gene was developed. The introduced mini-exon gene, which had been marked with a 40 bp tag, yielded the predicted tagged transcript. An upstream cis-acting element that was essential for transcription of the mini-exon gene was identified by site-directed mutagenesis. Block substitution mutagenesis of the -1 to +9 and +10 to +19 regions of the exon results in 20- to 100-fold decreased levels of the tagged transcript in steady-state RNA. However, since these two mutations resulted in only a 2- to 3-fold decrease in nascent RNA levels, steady-state levels appear to be affected greatest by the stability of the resulting transcript. In contrast, mutation of the -67/-58 region resulted in undetectable levels of both steady-state and nascent RNA from the introduced gene. We conclude, therefore, that this upstream element, which is highly conserved in all Leishmania species, is a component of the mini-exon gene promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.