ArticleThe EMBO journal1994
In vitro generation of lymphoid precursors from embryonic stem cells.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Generation and clinical potential of functional T lymphocytes from gene-edited pluripotent stem cells.Experimental hematology & oncology · 2022Review
- Visualizing structure and transitions in high-dimensional biological data.Nature biotechnology · 2019Article
- A PDMS-Based Microfluidic Hanging Drop Chip for Embryoid Body Formation.Molecules (Basel, Switzerland) · 2016Article
- The histone deacetylase inhibitor sodium valproate causes limited transcriptional change in mouse embryonic stem cells but selectively overrides Polycomb-mediated Hoxb silencing.Epigenetics & chromatin · 2013Article
- Experimental limitations using reprogrammed cells for hematopoietic differentiation.Journal of biomedicine & biotechnology · 2011Review
- Induction of hematopoietic differentiation of mouse embryonic stem cells by an AGM-derived stromal cell line is not further enhanced by overexpression of HOXB4.Stem cells and development · 2010Article
- Dosage compensation in the mouse balances up-regulation and silencing of X-linked genes.PLoS biology · 2007Article
- Characterization of developmental pathway of natural killer cells from embryonic stem cells in vitro.PloS one · 2007Article
- High-mobility-group A1 (HMGA1) proteins down-regulate the expression of the recombination activating gene 2 (RAG2).The Biochemical journal · 2005Article
- Biomedical and agricultural applications of animal transgenesis.Molecular biotechnology · 2004Review
- Isolation and therapeutic potential of human haemopoietic stem cells.Cytotechnology · 2003Article
- Development of hematopoietic cells from embryonic stem cells.International journal of hematology · 2001Review
- Functional characterization of B lymphocytes generated in vitro from embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Hemato-lymphoid in vivo reconstitution potential of subpopulations derived from in vitro differentiated embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Somatic-cell selection is a major determinant of the blood-cell phenotype in heterozygotes for glucose-6-phosphate dehydrogenase mutations causing severe enzyme deficiency.American journal of human genetics · 1996Article
- Oxygen supply and oxygen-dependent gene expression in differentiating embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- In vitro differentiation of murine embryonic stem cells. New approaches to old problems.The Journal of clinical investigation · 1996Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Murine embryonic stem (ES) cells represent a model system for studying certain aspects of hemopoiesis because they can differentiate in vitro into several cell types, including those of the hemopoietic system. We developed cell culture conditions in which ES cells undergo hemopoietic differentiation in a low-oxygen (5% O2) atmosphere without additional exogenous factors. After 15-20 days of culture under these conditions, cells bearing surface markers found on cells of the lymphoid lineage (Thy1+, Pgp-1+, c-kit+ and B-220+) were detected. After 13-15 days, transcripts for the recombinase activating genes (RAG) 1 and 2, interleukin (IL) 7, IL-7 receptor and c-kit were expressed. We also investigated rearrangements of the immunoglobulin (Ig) heavy and light chain and the T cell receptor (TCR) loci. After 15 days of differentiation, we detected DJH gene rearrangement with N-region diversity. Productive VHDJH rearrangements are found after 20 days, paralleled by V Kappa J Kappa recombinations indicating a developmental stage comparable, at least, with that of pre B cells. Rearrangements of TCR gamma as well as delta chain segments were also observed, but no TCR beta chain rearrangement. These results demonstrate that ES cells reproducibly generate lymphoid cells in vitro.
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