ArticleCurrent genetics1994
Multiple-copy integration in the yeast Yarrowia lipolytica.
Article in Current genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 231 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Engineering sweetness: zeocin-based strain screening to identify high-titerFood science and biotechnology · 2026Article
- Comparative Expression of Diacylglycerol Acyltransferases for Enhanced Accumulation of Punicic Acid-Enriched Triacylglycerols inMolecules (Basel, Switzerland) · 2026Article
- Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production.Microbial biotechnology · 2025Article
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Iterative gene integration mediated by 26S rDNA and non-homologous end joining for the efficient production of lycopene in Yarrowia lipolytica.Bioresources and bioprocessing · 2023Article
- Up Front Unfolded Protein Response Combined with Early Protein Secretion Pathway Engineering inInternational journal of molecular sciences · 2023Article
- Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts.Synthetic and systems biotechnology · 2023Review
- Article
- Biosynthesis of Fatty Acid Derivatives by RecombinantJournal of fungi (Basel, Switzerland) · 2023Article
- A yeast-based tool for screening mammalian diacylglycerol acyltransferase inhibitors.MicrobiologyOpen · 2022Article
- Development of Hypertolerant Strain ofACS sustainable chemistry & engineering · 2022Article
- Identification of genome integration sites for developing a CRISPR-based gene expression toolkit in Yarrowia lipolytica.Microbial biotechnology · 2022Article
- Enhancing very long chain fatty acids production in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Production of human milk fat substitute by engineered strains ofMetabolic engineering communications · 2022Article
- Establishment of genomic library technology mediated by non-homologous end joining mechanism in Yarrowia lipolytica.Science China. Life sciences · 2021Article
- Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway.Microbial biotechnology · 2021Article
- The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the YeastInternational journal of molecular sciences · 2021Article
- Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using an EcoRI-BglII fragment of the G unit of the rDNA of Y. lipolytica and a set of 11 deletions in the URA3 promoter, we have constructed several plasmids to test gene amplification in the rDNA. These plasmids contain the rDNA fragment for integration, defective versions of the URA3 gene, the XPR2 gene encoding alkaline extracellular protease (AEP) as a reporter gene, and part of the pBR322 plasmid for selection and replication in E. coli. Among these plasmids, one corresponds to a deletion which allows multiple integration into the rDNA (plasmid pINA773). Two other plasmids (pINA767 and pINA772) give multiple integration only with a mutated URA3 gene. Transformants carrying these three plasmids were tested for copy number, stability, chromosomal localization and AEP secretion. Transformants containing plasmids pINA767, 772 and 773 displayed an average copy number of 5, 12 and 25-60 copies respectively of the plasmid, as estimated by PCR and DNA hybridization. Integrations occurred in only one chromosome except for transformants containing 60 copies where copies were observed at least in two different chromosomes. Multiple integrations were found both as tandem repeats and as dispersed copies. Plasmid copy number was stable, in both minimum and rich media, for strains containing less than ten copies per cells. However, for higher copy number, multiple integrations were stable only when AEP synthesis was not induced, while in inducing medium stability of the multiple integrations was dramatically affected.
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