ArticleTransgenic research1994
High-level salivary gland expression in transgenic mice.
Article in Transgenic research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Protein-binding elements in the proximal parotid secretory protein gene enhancer essential for salivary-gland-specific expression.The Biochemical journal · 2001Article
- The main regulatory region in the murine PSP gene is a parotid gland enhancer.Transgenic research · 1998Article
- Novel salivary gland specific binding elements located in the PSP proximal enhancer core.Nucleic acids research · 1998Article
- Tissue-specific expression of human salivary mucin gene, MUC7, in transgenic mice.Transgenic research · 1998Article
- Characterization of the rat salivary-gland B1-immunoreactive proteins.The Biochemical journal · 1998Article
- Molecular cloning and characterization of the gene encoding rat submandibular gland apomucin, Mucsmg.Glycoconjugate journal · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 7.1 kb mini-gene construct containing cloned DNA from the murine parotid secretory protein (PSP) gene with 6.2 kb of the promoter, has previously been shown to direct specific mRNA expression to the salivary glands in transgenic mice. However, the level of transgene expression in the parotid gland was only a few percent of the endogenous level. This indicated that elements necessary for high-level expression are still to be found. In this study, we have searched for such regulatory elements in additional flanking regions by using a 25 kb cloned Pspb fragment containing the complete structural gene, 11.4 kb of 5'-flanking sequence, and 2.5 kb 3'-flanking sequence as a transgene. To distinguish the expression of the transgene from that of the endogenous gene, we took advantage of an allelic difference, using an oligonucleotide that recognized the mRNA from Pspb and the transgene but not that from the other allele, Pspa. The expression of the transgene was examined in animals homozygous for Pspa. Three independent integrations all exhibited a level of parotid-gland-specific expression that corresponded to that of the endogenous gene. Thus, sequences responsible for this high-level PSP mRNA expression are situated within the genomic DNA of the transgene.
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Registered trials
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