ArticleTransgenic research1994
Transcriptional and post-transcriptional events affect the production of secreted hen egg white lysozyme by Aspergillus niger.
Article in Transgenic research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 33 citations in OpenAlex.
- RoCi-a single-step multi-copy integration system based on rolling-circle replication.Nucleic acids research · 2026Article
- The C-Type Lysozyme from the upper Gastrointestinal Tract ofInternational journal of molecular sciences · 2019Article
- High-yield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure.Applied and environmental microbiology · 2003Article
- Characterization of a foldase, protein disulfide isomerase A, in the protein secretory pathway of Aspergillus niger.Applied and environmental microbiology · 2000Article
- Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels.Applied and environmental microbiology · 1997Article
- Glucose-induced secretion of Trichoderma reesei xylanases.Applied and environmental microbiology · 1996Article
- Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains.Applied and environmental microbiology · 1996Article
- Heterologous protein secretion by Aspergillus niger growing in submerged culture as dispersed or aggregated mycelia.Applied microbiology and biotechnology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relationship between heterologous gene copy number, mRNA and secreted protein yields has been studied in Aspergillus niger transformants containing either the hen egg-white lysozyme (HEWL) cDNA gene or a glucoamylase-HEWL gene fusion (incorporating the A. niger glaA gene). The results support a direct relationship between HEWL gene copy number, mRNA and secreted HEWL protein levels at low (< 25) copy numbers. High protein yields are associated with multiple copies of the recombinant gene at a single site. Fusion of the HEWL gene to the glucoamylase gene resulted in higher steady-state levels of heterologous mRNA. Transformants with the HEWL cDNA alone exhibited a ten-fold higher mRNA:protein ratio than transformants with the gene fusion indicating that post-transcriptional events significantly affect final secreted protein yields.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.