ArticleMolecular biology of the cell1994
Characterization of DLC-A and DLC-B, two families of cytoplasmic dynein light chain subunits.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- The yeast dynein Dyn2-Pac11 complex is a dynein dimerization/processivity factor: structural and single-molecule characterization.Molecular biology of the cell · 2013Article
- Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning.Molecular biology of the cell · 2011Article
- Neurodegenerative mutation in cytoplasmic dynein alters its organization and dynein-dynactin and dynein-kinesin interactions.The Journal of biological chemistry · 2010Article
- Dynein light intermediate chain: an essential subunit that contributes to spindle checkpoint inactivation.Molecular biology of the cell · 2008Article
- Genetic analysis of the cytoplasmic dynein subunit families.PLoS genetics · 2006Article
- Cytoplasmic dynein nomenclature.The Journal of cell biology · 2005Article
- GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis.Infection and immunity · 2005Article
- The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation.Molecular biology of the cell · 2004Article
- Analysis of the dynein-dynactin interaction in vitro and in vivo.Molecular biology of the cell · 2003Article
- A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in chlamydomonas and Mammalian cells.Molecular biology of the cell · 2003Article
- Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes.The Journal of cell biology · 2002Article
- Backbone dynamics of the 8 kDa dynein light chain dimer reveals molecular basis of the protein's functional diversity.Journal of biomolecular NMR · 2002Article
- Subunit organization in cytoplasmic dynein subcomplexes.Protein science : a publication of the Protein Society · 2002Article
- Identification of a novel light intermediate chain (D2LIC) for mammalian cytoplasmic dynein 2.Molecular biology of the cell · 2002Article
- Cytoplasmic dynein light intermediate chain is required for discrete aspects of mitosis in Caenorhabditis elegans.Molecular biology of the cell · 2001Article
- Subunit heterogeneity of cytoplasmic dynein: Differential expression of 14 kDa dynein light chains in rat hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization.The Journal of cell biology · 1999Article
- Increased protein phosphorylation of cytoplasmic dynein results in impaired motor function.The Biochemical journal · 1999Article
- Identification of the t complex-encoded cytoplasmic dynein light chain tctex1 in inner arm I1 supports the involvement of flagellar dyneins in meiotic drive.The Journal of cell biology · 1998Article
- Phosphoregulation of an inner dynein arm complex in Chlamydomonas reinhardtii is altered in phototactic mutant strains.The Journal of cell biology · 1997Article
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Authors and funding
3 authors.
Funding
Abstract
Cytoplasmic dynein is a minus-end-directed, microtubule-dependent motor composed of two heavy chains (approximately 530 kDa), three intermediate chains (approximately 74 kDa), and a family of approximately 52-61 kDa light chains. Although the approximately 530 kDa subunit contains the motor and microtubule binding domains of the complex, the functions of the smaller subunits are not known. Using two-dimensional gel electrophoresis and proteolytic mapping, we show here that the light chains are composed of two major families, a higher M(r) family (58, 59, 61 kDa; dynein light chain group A [DLC-A]) and lower M(r) family (52, 53, 55, 56 kDa; dynein light chain group B [DLC-B]). Dissociation of the cytoplasmic dynein complex with potassium iodide reveals that all light chain polypeptides are tightly associated with the approximately 530 kDa heavy chain, whereas the approximately 74 kDa intermediate chain polypeptides are more readily extracted. Treatment with alkaline phosphatase alters the mobility of four of the light chain polypeptides, indicating that these subunits are phosphorylated. Sequencing of a cDNA clone encoding one member of the DLC-A family reveals a predicted globular structure that is not homologous to any known protein but does contain numerous potential phosphorylation sites and a consensus nucleotide-binding motif.
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