ArticleBiophysical journal1994
Pulsed laser imaging of rapid Ca2+ gradients in excitable cells.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Precise Time Superresolution by Event Correlation Microscopy.Biophysical journal · 2019Article
- v-SNARE function in chromaffin cells.Pflugers Archiv : European journal of physiology · 2018Review
- How does the stimulus define exocytosis in adrenal chromaffin cells?Pflugers Archiv : European journal of physiology · 2018Review
- Heterogeneous distribution of exocytotic microdomains in adrenal chromaffin cells resolved by high-density diamond ultra-microelectrode arrays.The Journal of physiology · 2014Article
- Spatial resolution of single-cell exocytosis by microwell-based individually addressable thin film ultramicroelectrode arrays.Analytical chemistry · 2014Article
- Comparison of myoplasmic calcium movements during excitation-contraction coupling in frog twitch and mouse fast-twitch muscle fibers.The Journal of general physiology · 2013Article
- Inhibition of N and PQ calcium channels by calcium entry through L channels in chromaffin cells.Pflugers Archiv : European journal of physiology · 2009Article
- Observation of calcium microdomains at the uropod of living morphologically polarized human neutrophils using flash lamp-based fluorescence microscopy.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Article
- Measurement of changes in membrane surface morphology associated with exocytosis using scanning ion conductance microscopy.Biophysical journal · 2006Article
- Guiding neuronal growth cones using Ca2+ signals.Trends in cell biology · 2004Review
- Spatial distribution of Ca(2+) signals during repetitive depolarizing stimuli in adrenal chromaffin cells.Biophysical journal · 2003Article
- Physiological stimuli evoke two forms of endocytosis in bovine chromaffin cells.The Journal of physiology · 2001Article
- Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila.Genes & development · 2001Article
- Development and dissipation of Ca(2+) gradients in adrenal chromaffin cells.Biophysical journal · 2000Article
- Sarcomeric Ca2+ gradients during activation of frog skeletal muscle fibres imaged with confocal and two-photon microscopy.The Journal of physiology · 2000Article
- Nutrient modulation of polarized and sustained submembrane Ca2+ microgradients in mouse pancreatic islet cells.The Journal of physiology · 2000Article
- Novel action of BAPTA series chelators on intrinsic K+ currents in rat hippocampal neurones.The Journal of physiology · 2000Article
- Spatial Ca(2+) distribution in contracting skeletal and cardiac muscle cells.Biophysical journal · 2000Article
- Measurement of action potential-induced presynaptic calcium domains at a cultured neuromuscular junction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Pulsed laser imaging of Ca(2+) influx in a neuroendocrine terminal.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Excitable cells are thought to respond to action potentials by forming short lived and highly localized Ca2+ gradients near sites of Ca2+ entry or near the site of Ca2+ release by intracellular stores. However, conventional imaging techniques lack the spatial and temporal resolution to capture these gradients. Here we demonstrate the use of pulsed-laser microscopy to measure Ca2+ gradients with submicron spatial resolution and millisecond time resolution in two preparations where the Ca2+ signal is thought to be fast and highly localized: adrenal chromaffin cells, where the entry of Ca2+ through voltage dependent Ca2+ channels triggers exocytotic fusion; and skeletal muscle fibers, where intracellular Ca2+ release from the sarcoplasmic reticulum initiates contraction. In chromaffin cells, Ca2+ gradients developed over 10-100 ms and were initially restricted to discrete submembrane domains, or hot spots, before developing into complete rings of elevated Ca2+ concentration. In frog skeletal muscle large, short-lived (approximately 6 ms) Ca2+ gradients were observed within individual sarcomeres following induction of action potentials. The pulsed laser imaging approach permits, for the first time, the capture and critical examination of rapid Ca2+ signaling events such as those underlying excitation-secretion and excitation-contraction coupling.
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