ArticleThe Biochemical journal1994
Cloning and characterization of the human osteopontin gene and its promoter.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.
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Who cites it
31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 116 citations in OpenAlex.
- Prognostic Value of Osteopontin Splice Variant-c Expression in Breast Cancers: A Meta-Analysis.BioMed research international · 2016Pooled it
- Pooled it
- Immunohistochemical evaluation of osteopontin expression in triple-negative breast cancer.Archives of medical science : AMS · 2024Article
- Osteopontin as a Regulator of Colorectal Cancer Progression and Its Clinical Applications.Cancers · 2021Review
- Molecular Characterization and Expression ofGenes · 2019Article
- Review
- Induction of Osteopontin by Dengue Virus-3 Infection in THP-1 Cells: Inhibition of the Synthesis by Brefelamide and Its Derivative.Frontiers in microbiology · 2017Article
- Targeting of the leukemia microenvironment by c(RGDfV) overcomes the resistance to chemotherapy in acute myeloid leukemia in biomimetic polystyrene scaffolds.Oncology letters · 2016Article
- Article
- Osteopontin: A novel regulator at the cross roads of inflammation, obesity and diabetes.Molecular metabolism · 2014Review
- Mechanism of hepatitis C virus (HCV)-induced osteopontin and its role in epithelial to mesenchymal transition of hepatocytes.The Journal of biological chemistry · 2013Article
- Regulatory role of osteopontin in malignant transformation of endometrial cancer.Molecular biology reports · 2013Article
- New insights into the pathogenesis of pseudoxanthoma elasticum and related soft tissue calcification disorders by identifying genetic interactions and modifiers.Frontiers in genetics · 2013Article
- Osteopontin splice variants differentially exert clinicopathological features and biological functions in gastric cancer.International journal of biological sciences · 2013Article
- Osteopontin is overexpressed in colorectal carcinoma and is correlated with P53 by immunohistochemistry.Experimental and therapeutic medicine · 2012Article
- Pre- and post-translational regulation of osteopontin in cancer.Journal of cell communication and signaling · 2011Article
- Transcription factor c-Myb promotes the invasion of hepatocellular carcinoma cells via increasing osteopontin expression.Journal of experimental & clinical cancer research : CR · 2010Article
- A humanized anti-osteopontin antibody inhibits breast cancer growth and metastasis in vivo.Cancer immunology, immunotherapy : CII · 2010Article
- Identification of osteopontin-dependent signaling pathways in a mouse model of human breast cancer.BMC research notes · 2009Article
- Phosphorylated proteins and control over apatite nucleation, crystal growth, and inhibition.Chemical reviews · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated the human osteopontin (hOP) gene and the 5' upstream region, and analysed its exon-intron structure and potential regulatory sequences of the promoter region in comparison with those of the mouse and porcine gene. The coding sequence is split into 7 exons which are similar to those of the mouse gene, although the hOP gene is longer than the mouse gene. The difference in length is mainly due to variations in intron 3, which is approximately 2.7-fold longer than that of the mouse OP gene. The 5' upstream region of the hOP, which is highly conserved up to nucleotide -250, contains a number of potential cis regulatory consensus sequences. A series of sequentially 5'-deleted chimeric clones was tested for the ability to stimulate chloramphenicol acetyltransferase (CAT). Initial CAT analysis demonstrated that nucleotides at positions -474 to -270, -124 to -80, and -55 to -39 contained cis-acting enhancing sequences in a human monocyte cell line, SCC-3, although the -124 to -80 region was much more active than other regions. Deletion of the sequences between -474 and -270 localized this cis region to the sequence at positions -439 to -410, whereas the deletion between -124 to -80 localized the regions to -124 to -115, and -94 to -80. Gel-shift analysis using as probes synthesized double-stranded DNA corresponding to the 10 and 15 bp region at positions -124 to -115 and -94 to -80 respectively revealed that each probe formed a major band complexed with nuclear proteins prepared from SCC-3 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.