ArticleApplied and environmental microbiology1994
Synthetic peptides derived from the Listeria monocytogenes p60 protein as antigens for the generation of polyclonal antibodies specific for secreted cell-free L. monocytogenes p60 proteins.
Article in Applied and environmental microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection ofVeterinary world · 2025Article
- Harnessing immunopeptidomics for next-generation vaccines against intracellular bacterial pathogens.Molecular biology reports · 2025Review
- Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity.Frontiers in molecular biosciences · 2018Article
- Reliable and rapid identification of Listeria monocytogenes and Listeria species by artificial neural network-based Fourier transform infrared spectroscopy.Applied and environmental microbiology · 2006Article
- Morphotypic conversion in Listeria monocytogenes biofilm formation: biological significance of rough colony isolates.Applied and environmental microbiology · 2004Article
- Use of monoclonal antibodies that recognize p60 for identification of Listeria monocytogenes.Clinical and diagnostic laboratory immunology · 2004Article
- Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods.Applied and environmental microbiology · 2002Article
- Positive selection of mutations leading to loss or reduction of transcriptional activity of PrfA, the central regulator of Listeria monocytogenes virulence.Journal of bacteriology · 2001Article
- Listeria pathogenesis and molecular virulence determinants.Clinical microbiology reviews · 2001Review
- Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein.Journal of clinical microbiology · 2000Article
- Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR.Applied and environmental microbiology · 1999Article
- Generation of polyclonal antibodies of predetermined specificity against pediocin PA-1.Applied and environmental microbiology · 1998Article
- Unstable expression and thermal instability of a species-specific cell surface epitope associated with a 66-kilodalton antigen recognized by monoclonal antibody EM-7G1 within serotypes of Listeria monocytogenes grown in nonselective and selective broths.Applied and environmental microbiology · 1998Article
- Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus.Applied and environmental microbiology · 1998Article
- Evaluation of luciferase reporter bacteriophage A511::luxAB for detection of Listeria monocytogenes in contaminated foods.Applied and environmental microbiology · 1997Article
- Isolation of catalase-negative Listeria monocytogenes strains from listeriosis patients and their rapid identification by anti-p60 antibodies and/or PCR.Journal of clinical microbiology · 1997Article
- Catalase-negative Listeria monocytogenes causing lethal sepsis and meningitis in an adult hematologic patient.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1996Article
- Construction of luciferase reporter bacteriophage A511::luxAB for rapid and sensitive detection of viable Listeria cells.Applied and environmental microbiology · 1996Article
- A simple colony-blot method for identification of Listeria in food samples.Medical microbiology and immunology · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All species of the genus Listeria secrete a major extracellular protein called p60. A comparison of the deduced amino acid sequences of all listerial p60 proteins previously indicated there were only a few regions which were unique to the pathogenic, food-borne species Listeria monocytogenes. Two of these p60 regions were chosen for the development of antibodies specific for the facultative intracellular species L. monocytogenes. Initially, these regions were characterized via epitope mapping, and this led to the development of two different synthetic peptides. Rabbits immunized with these synthetic peptides generated polyclonal antibodies that were then used in Western blot (immunoblot) analyses. Antiserum against peptide A (PepA) recognized the p60 protein in the supernatants collected from most L. monocytogenes serotypes except for several strains belonging to serotypes 4a and 4c. No p60-related protein was detected in the supernatants from other Listeria species with this anti-PepA antiserum. Antibodies raised against peptide D (PepD) reacted with p60 from all L. monocytogenes serotypes, including all 4a and 4c strains that were tested, and also showed no cross-reactivity with supernatant proteins from other Listeria species. Both antisera also detected p60 in supernatants of a large number of environmental isolates of L. monocytogenes. Besides Western blot analyses, these antisera to PepA and PepD reacted with secreted p60 in an enzyme-linked immunosorbent assay, indicating recognition of the native antigen in addition to the denatured form. These data suggest that synthetic peptides derived from the variable region of the L. monocytogenes p60 protein may be useful for the development of an immunological diagnostic assay.
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