ArticleProceedings of the National Academy of Sciences of the United States of America1994
Expression of cAMP-dependent protein kinase in prespore cells is sufficient to induce spore cell differentiation in Dictyostelium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 72 citations in OpenAlex.
- Autophagy of the somatic stalk cells likely nurses the propagating spores of Dictyostelid social amoebas.Open research Europe · 2022Article
- Novel RNAseq-Informed Cell-type Markers and Their Regulation Alter Paradigms ofFrontiers in cell and developmental biology · 2022Article
- Cyclic AMP induction of Dictyostelium prespore gene expression requires autophagy.Developmental biology · 2019Article
- Modulation of cyclic nucleotide-mediated cellular signaling and gene expression using photoactivated adenylyl cyclase as an optogenetic tool.Scientific reports · 2017Article
- A conserved signalling pathway for amoebozoan encystation that was co-opted for multicellular development.Scientific reports · 2015Article
- The cyclic AMP phosphodiesterase RegA critically regulates encystation in social and pathogenic amoebas.Cellular signalling · 2014Article
- Loss of cAMP-specific phosphodiesterase rescues spore development in G protein mutant in dictyostelium.Cellular signalling · 2014Article
- Mef2A, a homologue of animal Mef2 transcription factors, regulates cell differentiation in Dictyostelium discoideum.BMC developmental biology · 2013Article
- Role of the Skp1 prolyl-hydroxylation/glycosylation pathway in oxygen dependent submerged development of Dictyostelium.BMC developmental biology · 2012Article
- Evolutionary crossroads in developmental biology: Dictyostelium discoideum.Development (Cambridge, England) · 2011Review
- G{alpha}5 subunit-mediated signalling requires a D-motif and the MAPK ERK1 in Dictyostelium.Microbiology (Reading, England) · 2010Article
- cAMP production by adenylyl cyclase G induces prespore differentiation in Dictyostelium slugs.Development (Cambridge, England) · 2007Article
- Constitutively active protein kinase A disrupts motility and chemotaxis in Dictyostelium discoideum.Eukaryotic cell · 2003Article
- cAMP signaling in Dictyostelium. Complexity of cAMP synthesis, degradation and detection.Journal of muscle research and cell motility · 2002Review
- Identification and characterization of DdPDE3, a cGMP-selective phosphodiesterase from Dictyostelium.The Biochemical journal · 2001Article
- Role of PKA in the timing of developmental events in Dictyostelium cells.Microbiology and molecular biology reviews : MMBR · 1998Review
- An intersection of the cAMP/PKA and two-component signal transduction systems in Dictyostelium.The EMBO journal · 1998Article
- A two-component histidine kinase gene that functions in Dictyostelium development.The EMBO journal · 1996Article
- Evidence that a combined activator-repressor protein regulates Dictyostelium stalk cell differentiation.The EMBO journal · 1996Article
- Genetic networks that regulate development in Dictyostelium cells.Microbiological reviews · 1996Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activity of cAMP-dependent protein kinase (PKA) is required for proper development at several stages during the Dictyostelium life cycle. We present evidence that activation of PKA is rate-limiting for the differentiation of prespore cells to spores and that PKA activation may be the developmental trigger for sporulation. Strains that overexpress the gene encoding the catalytic subunit of PKA (PKAcat) or lack a functional regulatory subunit (rdeC strains) undergo rapid, heterochronic development. We show that overexpression of PKAcat in prespore cell is sufficient to directly induce expression of the spore maturation marker spiA and differentiation to spores, in a cell-autonomous manner. Moreover, overexpression of PKAcat in prespore cells can bypass a mutation that blocks an earlier developmental step to induce spiA expression. Our results suggest that the regulatory pathway in prespore cells between the activation of PKA and spiA induction/spore maturation is quite short and that PKAcat expression in prespore cells may mediate spore differentiation at the level of transcription. This induction of sporulation requires the prior activation of the prespore cell pathway. In addition, we show that beta-galactosidase activity expressed from a PKAcat promoter/lacZ reporter construct is highly enriched in the anterior prestalk A region during the tipped aggregate, slug, and early culminant stages and that this pattern switches abruptly to a prespore pattern at the time of spore maturation, supporting the proposed role of PKA in this process.
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