ArticleProceedings of the National Academy of Sciences of the United States of America1994
Ankyrin and beta-spectrin accumulate independently of alpha-spectrin in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 64 citations in OpenAlex.
- A developmental pathway for epithelial-to-motoneuron transformation in C. elegans.Cell reports · 2022Article
- Human ankyrins and their contribution to disease biology: An update.Journal of biosciences · 2020Review
- Expression of a Fragment of Ankyrin 2 Disrupts the Structure of the Axon Initial Segment and Causes Axonal Degeneration in Drosophila.Molecular neurobiology · 2019Article
- A Presynaptic Function of Shank Protein inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Article
- Heterotrimeric Go protein links Wnt-Frizzled signaling with ankyrins to regulate the neuronal microtubule cytoskeleton.Development (Cambridge, England) · 2014Article
- Genetic studies of spectrin in the larval fat body of Drosophila melanogaster: evidence for a novel lipid uptake apparatus.Genetics · 2013Article
- Transgene rescue identifies an essential function for Drosophila beta spectrin in the nervous system and a selective requirement for ankyrin-2-binding activity.Molecular biology of the cell · 2010Article
- The Drosophila Anion Exchanger (DAE) lacks a detectable interaction with the spectrin cytoskeleton.Journal of negative results in biomedicine · 2010Article
- Membrane domains based on ankyrin and spectrin associated with cell-cell interactions.Cold Spring Harbor perspectives in biology · 2009Review
- The interaction between L1-type proteins and ankyrins--a master switch for L1-type CAM function.Cellular & molecular biology letters · 2009Review
- Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton.The Journal of biological chemistry · 2008Article
- Article
- A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction.The Journal of cell biology · 2006Article
- Spectrin functions upstream of ankyrin in a spectrin cytoskeleton assembly pathway.The Journal of cell biology · 2006Article
- Functional links between membrane transport and the spectrin cytoskeleton.The Journal of membrane biology · 2006Review
- The L1-type cell adhesion molecule neuroglian influences the stability of neural ankyrin in the Drosophila embryo but not its axonal localization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Structural requirements for outside-in and inside-out signaling by Drosophila neuroglian, a member of the L1 family of cell adhesion molecules.The Journal of cell biology · 1998Article
- Fate of dot chromosome genes in Drosophila willistoni and Scaptodrosophila lebanonensis determined by in situ hybridization.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
- Segregation of two spectrin isoforms: polarized membrane-binding sites direct polarized membrane skeleton assembly.Molecular biology of the cell · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the identification and initial characterization of Drosophila melanogaster ankyrin. Oligonucleotide primers based on the spectrin-binding domain of human brain ankyrin were used to amplify Drosophila genomic DNA. A cloned 184-bp PCR product was used to isolate Drosophila ankyrin cDNAs. Ankyrin cDNA probes detected a 5.5-kb transcript from embryonic poly(A)+ RNA and a single polytene chromosome locus (101F-102A). The cDNA sequence encodes a 170-kDa protein that is 53% identical to human brain ankyrin (Ank2). Antibodies directed against a recombinant fragment of Drosophila ankyrin reacted with a 170-kDa polypeptide from Drosophila embryos, larvae, S2 cells, and adult flies. The ankyrin antibody coimmunoprecipitated alpha- and beta-spectrin with ankyrin in detergent extracts of Drosophila embryo membranes. Antibodies against Drosophila ankyrin, alpha-spectrin and beta-spectrin were used to detect these proteins in wild-type and alpha-spectrin-mutant larvae. alpha-Spectrin levels were greatly diminished in mutant larvae, but levels of ankyrin and beta-spectrin were indistinguishable from wild type. The persistence of ankyrin and beta-spectrin may explain the relatively mild phenotype of alpha-spectrin mutants during early Drosophila development.
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