ArticleProceedings of the National Academy of Sciences of the United States of America1994
The primer tRNA sequence is not inherited during Ty1 retrotransposition.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Reverse Transcription in the Saccharomyces cerevisiae Long-Terminal Repeat Retrotransposon Ty3.Viruses · 2017Review
- Specific recognition and cleavage of the plus-strand primer by reverse transcriptase.Journal of virology · 2005Article
- Ty1 defect in proteolysis at high temperature.Journal of virology · 2002Article
- Extreme structural heterogeneity among the members of a maize retrotransposon family.Genetics · 1998Article
- In vivo Ty1 reverse transcription can generate replication intermediates with untidy ends.Journal of virology · 1998Article
- Extended minus-strand DNA as template for R-U5-mediated second-strand transfer in recombinational rescue of primer binding site-modified retroviral vectors.Journal of virology · 1998Article
- Replication errors during in vivo Ty1 transposition are linked to heterogeneous RNase H cleavage sites.Molecular and cellular biology · 1998Article
- Plus-strand strong-stop DNA transfer in yeast Ty retrotransposons.The EMBO journal · 1997Article
- Primer tRNAs for reverse transcription.Journal of virology · 1997Review
- Increased length of long terminal repeats inhibits Ty1 transposition and leads to the formation of tandem multimers.Genetics · 1997Article
- Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA.Journal of virology · 1996Article
- Yeast retrotransposon Ty4: the majority of the rare transcripts lack a U3-R sequence.Nucleic acids research · 1996Article
- ts1-Induced spongiform encephalomyelopathy: physical forms of high-mobility DNA in spinal cord tissues of paralyzed mice are products of premature termination of reverse transcription.Journal of virology · 1996Article
- Plus-strand strong-stop DNA synthesis in retrotransposon Ty1.Journal of virology · 1995Article
- Multiple molecular determinants for retrotransposition in a primer tRNA.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast retrotransposon Ty1 transposes through an RNA intermediate by a mechanism resembling retroviral replication. Long terminal repeat retroelements require primers for initiation of reverse transcription. The primer for minus-strand DNA synthesis is the 3' end of a cellular tRNA that base pairs to the complementary region of genomic RNA (the primer binding site). The genomic RNA of retroviruses and retrotransposons is shorter than its proviral DNA counterpart, lacking complete long terminal repeats. A variety of models have been proposed to describe how complete long terminal repeats are regenerated during reverse transcription. A common feature of these models is the requirement that the 3' portion of the primer tRNA be reverse-transcribed and then utilized in a strand-transfer reaction. We introduced a silent mutation into the Ty1 primer binding site and followed its fate during a single cycle of reverse transcription to directly test this aspect of the reverse transcription model. We demonstrate that the tRNA sequence is not inherited by progeny Ty1 elements during reverse transcription.
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