ArticleProceedings of the National Academy of Sciences of the United States of America1994
Imaging nuclear pores of aldosterone-sensitive kidney cells by atomic force microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Differences and similarities in biophysical and biological characteristics between U87 MG glioblastoma and astrocyte cells.Histochemistry and cell biology · 2024Article
- Physics of the Nuclear Pore Complex: Theory, Modeling and Experiment.Physics reports · 2021Article
- Characterization of Single-Nucleus Electrical Properties by Microfluidic Constriction Channel.Micromachines · 2019Article
- Article
- Atomic force microscopy reveals structural variability amongst nuclear pore complexes.Life science alliance · 2018Article
- 3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.Histochemistry and cell biology · 2012Article
- Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology.Pflugers Archiv : European journal of physiology · 2008Review
- Route of glucocorticoid-induced macromolecules across the nuclear envelope as viewed by atomic force microscopy.Pflugers Archiv : European journal of physiology · 2006Review
- Article
- Transient permeability leak of nuclear envelope induced by aldosterone.The Journal of membrane biology · 2004Article
- Nuclear envelope: nanoarray responsive to aldosterone.The Journal of membrane biology · 2004Review
- Modulation of nuclear pore topology by transport modifiers.Biophysical journal · 2003Article
- Aldosterone signaling pathway across the nuclear envelope.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Nuclear hourglass technique: an approach that detects electrically open nuclear pores in Xenopus laevis oocyte.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Atomic force microscopy visualizes ATP-dependent dissociation of multimeric TATA-binding protein before translocation into the cell nucleus.Pflugers Archiv : European journal of physiology · 1996Article
- Aldosterone activates the nuclear pore transporter in cultured kidney cells imaged with atomic force microscopy.Pflugers Archiv : European journal of physiology · 1996Article
- Single nuclear pores visualized by confocal microscopy and image processing.Biophysical journal · 1996Article
- Polarized ion transport during migration of transformed Madin-Darby canine kidney cells.Pflugers Archiv : European journal of physiology · 1995Article
- Immunolocalization of lamins and nuclear pore complex proteins by atomic force microscopy.Pflugers Archiv : European journal of physiology · 1995Article
- Patch clamp and atomic force microscopy demonstrate TATA-binding protein (TBP) interactions with the nuclear pore complex.The Journal of membrane biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In nuclei of renal target cells, aldosterone enhances transcriptional activity followed by the translocation of specific RNA molecules across the nuclear envelope. Trafficking between cell nucleus and cytoplasm occurs via nuclear pore complexes (NPCs) located in the double-layered nuclear envelope. We investigated the nucleocytoplasmic transport route by structure-function analysis at subcellular level in quiescent and aldosterone-stimulated cells. With atomic-force microscopy (AFM) we imaged individual pores of the nuclear surface of cultured kidney cells and related the number of pores per micron2 to nuclear envelope conductance (Gn, per micron2) evaluated electrically by current injection into the isolated nucleus. NPCs were equally distributed resembling "donut-like" structures with outer diameters of 134 +/- 12 nm (n = 50), each equipped with a central channel. Six hours of aldosterone exposure (0.1 microM) increased the number of NPCs per micron 2 of nuclear surface from 7.4 +/- 0.4 to 9.8 +/- 0.4 (n = 12; P < 0.01). At the same time Gn rose from 6900 +/- 520 to 9600 +/- 610 pS/micron2 paralleled by an increase of the intranuclear electrical potential from -2.8 +/- 0.2 to -6.2 +/- 0.4 mV (n = 18; P < 0.01). Assuming that NPCs represent the sole conductive pathway in the nuclear envelope, we calculate a mean single NPC conductance of 932 and 980 pS, in the absence and presence of aldosterone, respectively. We conclude that aldosterone facilitates nucleocytoplasmic transport by increasing the number of NPCs but not by modifying their biophysical properties. Possibly, aldosterone controls similar transport mechanisms in both plasma membrane and nuclear envelope.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.