ArticleProceedings of the National Academy of Sciences of the United States of America1994
Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.Nucleus (Austin, Tex.) · 2026Article
- Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.bioRxiv : the preprint server for biology · 2026Article
- Article
- Atg39 selectively captures inner nuclear membrane into lumenal vesicles for delivery to the autophagosome.The Journal of cell biology · 2021Article
- Inducible intracellular membranes: molecular aspects and emerging applications.Microbial cell factories · 2020Review
- Chromosomal aneuploidies induced upon Lamin B2 depletion are mislocalized in the interphase nucleus.Chromosoma · 2017Article
- System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics.The Journal of cell biology · 2011Article
- Requirement for lamin B receptor and its regulation by importin {beta} and phosphorylation in nuclear envelope assembly during mitotic exit.The Journal of biological chemistry · 2010Article
- Nuclear pore biogenesis into an intact nuclear envelope.Chromosoma · 2010Review
- Farnesylated nuclear proteins Kugelkern and lamin Dm0 affect nuclear morphology by directly interacting with the nuclear membrane.Molecular biology of the cell · 2010Article
- Nuclear envelope-limited chromatin sheets (ELCS) and heterochromatin higher order structure.Chromosoma · 2009Review
- Generation of cubic membranes by controlled homotypic interaction of membrane proteins in the endoplasmic reticulum.The Journal of biological chemistry · 2009Article
- A-type nuclear lamins act as transcriptional repressors when targeted to promoters.Experimental cell research · 2009Article
- Granulocytic nuclear differentiation of lamin B receptor-deficient mouse EPRO cells.Experimental hematology · 2008Article
- Transcriptionally active PCR for antigen identification and vaccine development: in vitro genome-wide screening and in vivo immunogenicity.Molecular and biochemical parasitology · 2008Article
- Immobility, inheritance and plasticity of shape of the yeast nucleus.BMC cell biology · 2007Article
- Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion.Molecular biology of the cell · 2006Article
- Formation of stacked ER cisternae by low affinity protein interactions.The Journal of cell biology · 2003Article
- A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope.The Journal of cell biology · 2001Article
- A novel complex of membrane proteins required for formation of a spherical nucleus.The EMBO journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have expressed human lamin B and the chicken lamin B receptor (LBR), either separately or together, in yeast and have monitored the subcellular location of the expressed proteins by immunofluorescence microscopy, immunoelectron microscopy, and cell fractionation. At the light microscopic level, the heterologous lamin B localized to the yeast nuclear rim and at electron microscopic resolution was found subjacent to the yeast inner nuclear membrane. These data indicate that vertebrate lamin B was correctly targeted in yeast. Expression of the heterologous LBR, either alone or together with the heterologous lamin B, resulted in the formation of membrane stacks primarily adjacent to the nuclear envelope, but also projecting from the nuclear envelope into the cytoplasm or under the plasma membrane. Double immunoelectron microscopy showed colocalization of the heterologous lamin B and LBR in the yeast nuclear envelope and in the LBR-induced membrane stacks. Cell fractionation showed the presence of the heterologous lamin B and LBR in a subnuclear fraction enriched in nuclear envelopes. The heterologous lamin B was extracted at 8 M urea, but not at 4 M urea, thus behaving as a peripheral membrane protein and indistinguishable from assembled lamins. The heterologous LBR was not extracted by 8 M urea, indicating that it was integrated into the membrane. The observed colocalization and cofractionation are consistent with previously reported in vitro binding data and suggest that heterologous lamin B and LBR interact with each other when coexpressed in yeast.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.