ArticleProceedings of the National Academy of Sciences of the United States of America1994
Role of the cdc25C phosphatase in G2 arrest induced by nitrogen mustard.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 87 citations in OpenAlex.
- Activation of the DNA Damage Response by RNA Viruses.Biomolecules · 2016Review
- Cell cycle-dependent Cdc25C phosphatase determines cell survival by regulating apoptosis signal-regulating kinase 1.Cell death and differentiation · 2015Article
- The Inhibitory Effect of Buddlejasaponin IV on the Growth of YD-10B Human Oral Squamous Cell Carcinoma Cells.Journal of cancer prevention · 2013Article
- Induction of G2/M arrest by pseudolaric acid B is mediated by activation of the ATM signaling pathway.Acta pharmacologica Sinica · 2009Article
- Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents.Journal of molecular biology · 2007Article
- Article
- UCN-01 enhances the in vitro toxicity of clinical agents in human tumor cell lines.Investigational new drugs · 2000Article
- Repression of CDK1 and other genes with CDE and CHR promoter elements during DNA damage-induced G(2)/M arrest in human cells.Molecular and cellular biology · 2000Article
- The G(2) checkpoint is maintained by redundant pathways.Molecular and cellular biology · 1999Article
- Cell cycle control, checkpoint mechanisms, and genotoxic stress.Environmental health perspectives · 1999Review
- Cell-cycle arrest and apoptosis hypersusceptibility as a consequence of Lck deficiency in nontransformed T lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Nuclear localization of cyclin B1 controls mitotic entry after DNA damage.The Journal of cell biology · 1998Article
- The G2/M DNA damage checkpoint inhibits mitosis through Tyr15 phosphorylation of p34cdc2 in Aspergillus nidulans.The EMBO journal · 1997Article
- Effect of melphalan and hyperthermia on p34cdc2 kinase activity in human melanoma cells.British journal of cancer · 1996Article
- Competency in mismatch repair prohibits clonal expansion of cancer cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.The Journal of clinical investigation · 1996Article
- Stockpiling of Cdc25 during a DNA replication checkpoint arrest in Schizosaccharomyces pombe.Molecular and cellular biology · 1996Article
- p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Blocking Chk1 expression induces apoptosis and abrogates the G2 checkpoint mechanism.Neoplasia (New York, N.Y.)Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G2 arrest induced by nitrogen mustard in human lymphoma CA46 cells is associated with a failure to activate hyperphosphorylated cdc2/cyclin B1 complexes. We investigated the possibility that this might be due to a suppression of cdc25C phosphatase activity. cdc25C from interphase cells migrated as a 54- to 57-kDa doublet in SDS gels and exhibited basal phosphatase activity. cdc25C from mitotic cells migrated as a 66-kDa hyperphosphorylated species and exhibited elevated phosphatase activity. cdc25C hyperphosphorylation and activation were mediated by cdc2, supporting the view of a cdc2-cdc25C autocatalytic feedback loop. Immunofluorescence and cell fractionation studies suggested cdc2-cdc25C interaction occurred within the cytoplasm. Cells arrested in G2 phase following nitrogen mustard treatment or cells arrested in S phase with aphidicolin failed to dephosphorylate and activate cdc2, and this correlated with failure to convert cdc25C into the most active hyperphosphorylated species. Our findings suggest that checkpoints guarding against mitotic entry in the presence of unreplicated or damaged DNA suppress formation of the cdc2-cdc25C autocatalytic feedback loop that normally brings about rapid activation of cdc2.
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