ArticleNucleic acids research1994
Dissection of the locus control function located on the chicken lysozyme gene domain in transgenic mice.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 97 citations in OpenAlex.
- Maintenance of a functional higher order chromatin structure: The role of the nuclear matrix in normal and disease states.Gene therapy & molecular biology · 2009Article
- Developmental activation of the lysozyme gene in chicken macrophage cells is linked to core histone acetylation at its enhancer elements.Nucleic acids research · 2006Article
- Comparative analysis of chromatin landscape in regulatory regions of human housekeeping and tissue specific genes.BMC bioinformatics · 2005Article
- Biomedical and agricultural applications of animal transgenesis.Molecular biotechnology · 2004Review
- Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool.Microbiology and molecular biology reviews : MMBR · 2003Review
- Spatial organization of gene expression: the active chromatin hub.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Suppression of transgene silencing by matrix attachment regions in maize: a dual role for the maize 5' ADH1 matrix attachment region.The Plant cell · 2002Article
- A Functional chromatin domain does not resist X chromosome inactivation: silencing of cLys correlates with methylation of a dual promoter-replication origin.Molecular and cellular biology · 2002Article
- The chicken lysozyme chromatin domain contains a second, widely expressed gene.Nucleic acids research · 2002Article
- Chromatin fine structure profiles for a developmentally regulated gene: reorganization of the lysozyme locus before trans-activator binding and gene expression.Genes & development · 2000Article
- Use of matrix attachment regions (MARs) to minimize transgene silencing.Plant molecular biology · 2000Review
- Pre-selection of integration sites imparts repeatable transgene expression.Nucleic acids research · 2000Article
- Herpesvirus saimiri-based gene delivery vectors maintain heterologous expression throughout mouse embryonic stem cell differentiation in vitro.Gene therapy · 2000Article
- Position-independent expression of transgenes in zebrafish.Transgenic research · 1999Article
- Conservation of sequence and structure flanking the mouse and human beta-globin loci: the beta-globin genes are embedded within an array of odorant receptor genes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A new element within the T-cell receptor alpha locus required for tissue-specific locus control region activity.Molecular and cellular biology · 1999Article
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- The locus control region is necessary for gene expression in the human beta-globin locus but not the maintenance of an open chromatin structure in erythroid cells.Molecular and cellular biology · 1998Article
- Expression of human erythropoietin transgenes and of the endogenous WAP gene in the mammary gland of transgenic rabbits during gestation and lactation.Transgenic research · 1998Article
- The developmental activation of the chicken lysozyme locus in transgenic mice requires the interaction of a subset of enhancer elements with the promoter.Nucleic acids research · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The entire chicken lysozyme gene locus including all known cis-regulatory sequences and the 5' and 3' matrix attachment sites defining the borders of the DNase I sensitive chromatin domain, is expressed at a high level and independent of its chromosomal position in macrophages of transgenic mice. It was concluded that the lysozyme gene locus carries a locus control function. We analysed several cis-regulatory deletion mutants to investigate their influence on tissue specificity and level of expression. Position independent expression of the gene is lost whenever one of the upstream tissue specific enhancer regions is deleted, although tissue specific expression is usually retained. Deletion of the domain border fragments has no influence on copy number dependency of expression. However, without these regions an increased incidence of ectopic expression is observed. This suggests that the domain border fragments may help to suppress transgene expression in inappropriate tissues. We conclude, that position independent expression of the lysozyme gene is not controlled by a single specific region of the locus but is the result of the concerted action of several tissue specific upstream regulatory DNA elements with the promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.