ArticleNucleic acids research1994
Analysis of a 70 kb segment of DNA containing the human zeta and alpha-globin genes linked to their regulatory element (HS-40) in transgenic mice.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Chromatin loops, gene positioning, and gene expression.Frontiers in genetics · 2012Article
- A 191-kb genomic fragment containing the human alpha-globin locus can rescue alpha-thalassemic mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Complementation of alpha-thalassaemia in alpha-globin knockout mice with a 191 kb transgene containing the human alpha-globin locus.Transgenic research · 2004Article
- The control of expression of the alpha-globin gene cluster.International journal of hematology · 2002Review
- The expression of human alpha -like globin genes in transgenic mice mediated by bacterial artificial chromosome.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Identification of a conserved erythroid specific domain of histone acetylation across the alpha-globin gene cluster.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Loading of DNA-binding factors to an erythroid enhancer.Molecular and cellular biology · 2000Article
- A 3'-flanking NF-kappaB site mediates developmental silencing of the human zeta-globin gene.The EMBO journal · 1999Article
- Dividing the empire: boundary chromatin elements delimit the territory of enhancers.The EMBO journal · 1999Review
- Mechanisms of developmental control of transcription in the murine alpha- and beta-globin loci.Genes & development · 1999Article
- Derepression of human embryonic zeta-globin promoter by a locus-control region sequence.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Chromatin interaction mechanism of transcriptional control in vivo.The EMBO journal · 1998Article
- Sequence-specific ligation of DNA using RecA protein.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Characterization and localization of the mProx1 gene directly upstream of the mouse alpha-globin gene cluster: identification of a polymorphic direct repeat in the 5'UTR.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice.Nucleic acids research · 1996Article
- Age-dependent silencing of globin transgenes in the mouse.Nucleic acids research · 1996Article
- CACCC and GATA-1 sequences make the constitutively expressed alpha-globin gene erythroid-responsive in mouse erythroleukemia cells.Nucleic acids research · 1996Article
- Position-dependent variegation of globin transgene expression in mice.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Function of the upstream hypersensitive sites of the chicken beta-globin gene cluster in mice.Nucleic acids research · 1995Article
- Contrasting effects of alpha and beta globin regulatory elements on chromatin structure may be related to their different chromosomal environments.The EMBO journal · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have ligated two cosmids through an oligonucleotide linker to produce a single fragment spanning 70 kb of the human alpha-globin cluster, in which the alpha-like globin genes (zeta 2, alpha 2 and alpha 1), their regulatory element (HS-40) and erythroid-specific DNase I hypersensitive sites accurately retain their normal genomic organization. The zeta (embryonic) and alpha (embryonic, fetal and adult) globin genes were expressed in all 17 transgenic embryos. Similarly, all fetal and adult mice from seven transgenic lines that contained one or more copies of the fragment, produced up to 66% of the level of endogenous mouse alpha-globin mRNA. However, as for smaller constructs containing these elements, human alpha-globin expression was not copy number dependent and decreased by 1.5-9.0 fold during development. These findings suggest that either it is not possible to obtain full regulation of human alpha-globin expression in transgenic mice or, more likely, that additional alpha-globin regulatory elements lie beyond the 70 kb segment of DNA analysed.
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