ArticleNucleic acids research1994
The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Exploiting CRISPR-Cas9 technology to investigate individual histone modifications.Nucleic acids research · 2018Article
- Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions.PLoS neglected tropical diseases · 2011Article
- A 10 base-pair sequence within Domain III of the GPEET procyclin promoter is essential for the autonomous replication of a plasmid in procyclic Trypanosoma brucei.Molecular and biochemical parasitology · 2007Article
- Differential use of multiple replication origins in the ribosomal DNA episome of the protozoan parasite Entamoeba histolytica.Nucleic acids research · 2003Article
- Effect of large targeted deletions on the mitotic stability of an extra chromosome mediating drug resistance in Leishmania.Nucleic acids research · 2001Article
- Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Artificial linear mini-chromosomes for Trypanosoma brucei.Nucleic acids research · 1996Article
- Construction of trypanosome artificial mini-chromosomes.Nucleic acids research · 1995Article
- Control of gene expression in trypanosomes.Microbiological reviews · 1995Review
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
We have previously reported the construction and characterization of an autonomously replicating plasmid in Trypanosoma brucei. In this plasmid the procyclic acidic repetitive protein (PARP) gene promoter drives the transcription of a selectable marker. Deletion of this promoter incapacitates the plasmid, suggesting its utilization as a promoter-trap. Three independent libraries were created by inserting variously digested T.brucei genomic DNA into this promoterless construct. Transfection of these libraries into procyclic T.brucei and the subsequent isolation of episomes led only to the reisolation of the PARP promoter. Additionally, a ribosomal RNA promoter failed to keep the construct as an episome, although it can sustain mRNA transcription in T.brucei and was shown to be an efficient promoter in this construct. Finally, by using a transient replication assay involving the methylation-sensitive restriction endonuclease DpnI to distinguish between input and replicated DNA, we showed that the PARP promoter-bearing construct could replicate autonomously in procyclic T.brucei, but the corresponding construct with the rRNA promoter could not. The close association between elements that sustain transcription and DNA replication in T.brucei mirrors results observed in several higher eukaryotes and their viruses and suggests an ancient origin of this feature.
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