ArticleNucleic acids research1994
Production and characterization of recombinant human Ku antigen.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
- Amphiphilic Oligonucleotide Derivatives as a Tool to Study DNA Repair Proteins.International journal of molecular sciences · 2025Article
- The Ku complex promotes DNA end-bridging and this function is antagonized by Tel1/ATM kinase.Nucleic acids research · 2023Article
- Review
- DNA Double Strand Break Repair Pathways in Response to Different Types of Ionizing Radiation.Frontiers in genetics · 2021Review
- C-terminal region of bacterial Ku controls DNA bridging, DNA threading and recruitment of DNA ligase D for double strand breaks repair.Nucleic acids research · 2016Article
- XRCC4/XLF Interaction Is Variably Required for DNA Repair and Is Not Required for Ligase IV Stimulation.Molecular and cellular biology · 2015Article
- Mycobacterium tuberculosis Ku can bind to nuclear DNA damage and sensitize mammalian cells to bleomycin sulfate.Mutagenesis · 2011Article
- The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation.Molecular biology of the cell · 2011Article
- An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins.The Journal of biological chemistry · 2011Article
- Sequential loading of Saccharomyces cerevisiae Ku and Cdc13p to telomeres.The Journal of biological chemistry · 2009Article
- The Ku80 carboxy terminus stimulates joining and artemis-mediated processing of DNA ends.Molecular and cellular biology · 2009Article
- 2-Step purification of the Ku DNA repair protein expressed in Escherichia coli.Protein expression and purification · 2007Article
- Terminal DNA structure and ATP influence binding parameters of the DNA-dependent protein kinase at an early step prior to DNA synapsis.Nucleic acids research · 2006Article
- Visualization of inositol phosphate-dependent mobility of Ku: depletion of the DNA-PK cofactor InsP6 inhibits Ku mobility.Nucleic acids research · 2004Article
- Rad52 and Ku bind to different DNA structures produced early in double-strand break repair.Nucleic acids research · 2003Article
- In vivo association of Ku with mammalian origins of DNA replication.Molecular biology of the cell · 2001Article
- The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- DNA-binding and strand-annealing activities of human Mre11: implications for its roles in DNA double-strand break repair pathways.Nucleic acids research · 2001Article
- Molecular architecture of the mouse DNA polymerase alpha-primase complex.Molecular and cellular biology · 1999Article
- Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recombinant Ku have been largely unsuccessful. Here, we utilize two recombinant baculoviral vectors that carry p70 or p86 cDNA and express the Ku subunits individually as well as assemble them into the complete Ku heterodimer. In an electrophoretic mobility shift assay, recombinant Ku binds to linear double-stranded DNA but not to supercoiled, nicked circular, nor linear single-stranded DNA. Neither subunit binds DNA by itself indicating that heterodimerization is essential for function. We also describe a simple purification method for the isolation of highly purified recombinant Ku using a hexahistidine tag. The baculovirus expression system provides a stable and efficient source of not only the p70 and p86 subunits but also the functional Ku heterodimer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.