ArticleNucleic acids research1994
Polypyrimidine tract sequences direct selection of alternative branch sites and influence protein binding.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 75 citations in OpenAlex.
- Pervasive misannotation of microexons that are evolutionarily conserved and crucial for gene function in plants.Nature communications · 2022Article
- High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin.Oncotarget · 2017Article
- Cancer-associated SF3B1 mutations affect alternative splicing by promoting alternative branchpoint usage.Nature communications · 2016Article
- Sequence-specific flexibility organization of splicing flanking sequence and prediction of splice sites in the human genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Article
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- Intron definition and a branch site adenosine at nt 385 control RNA splicing of HPV16 E6*I and E7 expression.PloS one · 2012Article
- An SF1 affinity model to identify branch point sequences in human introns.Nucleic acids research · 2011Article
- Histone deacetylase activity modulates alternative splicing.PloS one · 2011Article
- Co-transcriptional splicing of constitutive and alternative exons.RNA (New York, N.Y.) · 2009Article
- Characterization of human GTPBP3, a GTP-binding protein involved in mitochondrial tRNA modification.Molecular and cellular biology · 2008Article
- hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.RNA (New York, N.Y.) · 2007Article
- Identification of alternative 5'/3' splice sites based on the mechanism of splice site competition.Nucleic acids research · 2006Article
- PTB regulates the processing of a 3'-terminal exon by repressing both splicing and polyadenylation.Molecular and cellular biology · 2005Article
- Exon repression by polypyrimidine tract binding protein.RNA (New York, N.Y.) · 2005Article
- Construction of a herpes simplex virus type 1 mutant with only a three-nucleotide change in the branchpoint region of the latency-associated transcript (LAT) and the stability of its two-kilobase LAT intron.Journal of virology · 2004Article
- A single polypyrimidine tract binding protein (PTB) binding site mediates splicing inhibition at mouse IgM exons M1 and M2.RNA (New York, N.Y.) · 2004Article
- The role of a common TNNT2 polymorphism in cardiac hypertrophy.Journal of human genetics · 2004Article
- A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG.The EMBO journal · 2003Article
- Mutations in RRM4 uncouple the splicing repression and RNA-binding activities of polypyrimidine tract binding protein.RNA (New York, N.Y.) · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IVS1, an intron derived from the rat fibronectin gene, is spliced inefficiently in vitro, involving the use of three alternative branch sites. Mutation of one branch point site, BP3, so as to increase complementarity to U2 snRNA resulted in exclusive use of that site and improved splicing efficiency, indicating that the wild type BP3 site is one determinant of poor IVS1 splicing. Deletions within the polypyrimidine tract had a variable effect on splicing efficiency and altered the pattern of branch site usage. Selection of each branch site was influenced negatively by purine substitutions ca. 20 nucleotides downstream. It is proposed that all three IVS1 branch sites are pyrimidine tract-dependent. Pyrimidine tract deletions also influenced the crosslinking of PTB (the polypyrimidine tract-binding protein), hnRNP C, and splicing factor U2AF65. All three proteins bound preferentially to distinct regions within the polypyrimidine tract and thus are candidates for mediating pyrimidine tract-dependent branch site selection. The findings indicate the complexity of the IVS1 polypyrimidine tract and suggest a crucial role for this region in modulating branch site selection and IVS1 splicing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.