ArticleNucleic acids research1994
Cloning and functional analysis of spliced isoforms of human nuclear factor I-X: interference with transcriptional activation by NFI/CTF in a cell-type specific manner.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 79 citations in OpenAlex.
- Identification of novel genes associated with HIV-1 latency by analysis of histone modifications.Human genomics · 2017Article
- Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds.Molecular biology reports · 2012Article
- Nuclear factor one transcription factors in CNS development.Molecular neurobiology · 2009Article
- Regulation of Cyp2a5 transcription in mouse primary hepatocytes: roles of hepatocyte nuclear factor 4 and nuclear factor I.The Biochemical journal · 2004Article
- Nuclear factor I/thyroid transcription factor 1 interactions modulate surfactant protein C transcription.Molecular and cellular biology · 2003Article
- The Nuclear Factor I (NFI) gene family in mammary gland development and function.Journal of mammary gland biology and neoplasia · 2003Review
- Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- JC virus multiplication in human hematopoietic progenitor cells requires the NF-1 class D transcription factor.Journal of virology · 2001Article
- A novel silencer element in the bovine papillomavirus type 4 promoter represses the transcriptional response to papillomavirus E2 protein.Journal of virology · 2001Article
- The differentiation-specific factor CDP/Cut represses transcription and replication of human papillomaviruses through a conserved silencing element.Journal of virology · 2000Article
- Differential binding of NF1 transcription factor to P53 gene promoter and its depletion in human breast tumours.Molecular biology reports · 1999Article
- Increased induction of osteopetrosis, but unaltered lymphomagenicity, by murine leukemia virus SL3-3 after mutation of a nuclear factor 1 site in the enhancer.Journal of virology · 1999Article
- Disruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Studies of the regulation of the mouse carboxyl ester lipase gene in mammary gland.The Biochemical journal · 1998Article
- A novel YY1-independent silencer represses the activity of the human papillomavirus type 16 enhancer.Journal of virology · 1998Article
- A combination of MEF3 and NFI proteins activates transcription in a subset of fast-twitch muscles.Molecular and cellular biology · 1997Article
- Second-site proviral enhancer alterations in lymphomas induced by enhancer mutants of SL3-3 murine leukemia virus: negative effect of nuclear factor 1 binding site.Journal of virology · 1997Article
- YY1 and NF1 both activate the human p53 promoter by alternatively binding to a composite element, and YY1 and E1A cooperate to amplify p53 promoter activity.Molecular and cellular biology · 1996Article
- YY1 represses human papillomavirus type 16 transcription by quenching AP-1 activity.Journal of virology · 1996Article
- CTF5--a new transcriptional activator of the NFI/CTF family.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies of the epithelial specificity of the human papillomavirus type 16 (HPV-16) enhancer pointed out an important role of nuclear factor I (NFI). In epithelial cells, NFI proteins are derived from the NFI-C gene and referred to as NFI/CTF. In contrast, fibroblasts, where the enhancer is inactive, express high levels of NFI from the NFI-X gene. To compare NFI-C and NFI-X derived transcription factors, we cloned and functionally investigated two differentially spliced forms of NFI-X from human fibroblasts. NFI-X1 has 95% homology with a transcript previously identified in hamster liver cells. NFI-X2, a spliced variant, misses 41 amino acids of the proline-rich activation domain. NFI-X expression, examined by Northern blots, shows strong cell-type specific variation in comparison with NFI/CTF. While the transcriptional activation domain of NFI-X2, functionally tested as GAL4-fusion protein in epithelial and fibroblast cells, activates transcription from promoter as well as enhancer position similar to NFI/CTF-1, the activation domain of NFI-X1 fails to activate transcription from enhancer position. In Drosophila cells, void of endogenous NFI proteins, full length NFI/CTF-1 and NFI-X2 activate a reporter construct containing only NFI sites as well as the NFI dependent HPV-16 enhancer. In contrast, NFI-X1 fails to activate the HPV-16 enhancer. Furthermore, overexpression of NFI-X1 in epithelial cells down-regulates the HPV-16 enhancer. Our findings suggest that the family of NFI transcription factors should not be viewed as constitutive activators, but rather, that NFI-C and NFI-X have divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.
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