ArticleNucleic acids research1994
The topoisomerase I gene from Ustilago maydis: sequence, disruption and mutant phenotype.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Review and Current Perspectives on DNA Topoisomerase I and II Enzymes of Fungi as Study Models for the Development of New Antifungal Drugs.Journal of fungi (Basel, Switzerland) · 2024Review
- Comparative Proteomic Analysis ofJournal of fungi (Basel, Switzerland) · 2022Article
- Protein Composition of Infectious Spores Reveals Novel Sexual Development and Germination Factors in Cryptococcus.PLoS genetics · 2015Article
- Topoisomerase I is essential in Cryptococcus neoformans: role In pathobiology and as an antifungal target.Genetics · 1999Article
- Interaction between Ustilago maydis REC2 and RAD51 genes in DNA repair and mitotic recombination.Genetics · 1997Article
- Assembly of nucleosomal DNA in a cell-free extract from wild-type and top1- strains of Ustilago maydis.Molecular & general genetics : MGG · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ustilago maydis genomic TOP1 gene encoding DNA topoisomerase I was cloned by amplifying a gene fragment using the polymerase chain reaction, and using this fragment to search a genomic DNA library by hybridization. The predicted peptide sequence exhibited 30-40% identity to other eukaryotic TOP1 genes, yet differed in several features. First, an unusually long acidic region was identified near the amino terminus (28/29 residues are acidic), which resembles other nucleolar peptide motifs. Second, an atypical carboxy-terminal 'tail', absent in other TOP1 genes, followed the active site tyrosine residue. A top1 gene disruption mutant was constructed by replacing the genomic TOP1 gene, with a top1::HygR null allele. This mutant lost the abundant topoisomerase I activity evident in wild-type U.maydis, and displayed a subtle coloration phenotype evident during cell senescence.
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Registered trials
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