ArticleMolecular and cellular biology1994
Identification and characterization by antisense oligonucleotides of exon and intron sequences required for splicing.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- A fluorescent splice-switching mouse model enables high-throughput, sensitive quantification of antisense oligonucleotide delivery and activity.Cell reports methods · 2024Article
- Analogues of Artificial Human Box C/D Small Nucleolar RNA As Regulators of Alternative Splicing of a pre-mRNA Target.Acta naturae · 2012Article
- Effects of splicing mutations on NF2-transcripts: transcript analysis and information theoretic predictions.Genes, chromosomes & cancer · 2011Article
- On future's doorstep: RNA interference and the pharmacopeia of tomorrow.The Journal of clinical investigation · 2007Review
- Exon ligation is proofread by the DExD/H-box ATPase Prp22p.Nature structural & molecular biology · 2006Article
- Construction of a herpes simplex virus type 1 mutant with only a three-nucleotide change in the branchpoint region of the latency-associated transcript (LAT) and the stability of its two-kilobase LAT intron.Journal of virology · 2004Article
- Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.Nucleic acids research · 2001Article
- Sensitivity of splice sites to antisense oligonucleotides in vivo.RNA (New York, N.Y.) · 1999Article
- Advantages of 2'-O-methyl oligoribonucleotide probes for detecting RNA targets.Nucleic acids research · 1998Article
- U1 small nuclear RNA-promoted exon selection requires a minimal distance between the position of U1 binding and the 3' splice site across the exon.Molecular and cellular biology · 1997Article
- Selection of a nonconsensus branch point is influenced by an RNA stem-loop structure and is important to confer stability to the herpes simplex virus 2-kilobase latency-associated transcript.Journal of virology · 1997Article
- Repair of thalassemic human beta-globin mRNA in mammalian cells by antisense oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Inverse splicing of a discontinuous pre-mRNA intron generates a circular exon in a HeLa cell nuclear extract.Nucleic acids research · 1996Article
- Pre-mRNA topology is important for 3'-end formation in Saccharomyces cerevisiae and mammals.Molecular and cellular biology · 1996Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Certain thalassemic human beta-globin pre-mRNAs carry mutations that generate aberrant splice sites and/or activate cryptic splice sites, providing a convenient and clinically relevant system to study splice site selection. Antisense 2'-O-methyl oligoribonucleotides were used to block a number of sequences in these pre-mRNAs and were tested for their ability to inhibit splicing in vitro or to affect the ratio between aberrantly and correctly spliced products. By this approach, it was found that (i) up to 19 nucleotides upstream from the branch point adenosine are involved in proper recognition and functioning of the branch point sequence; (ii) whereas at least 25 nucleotides of exon sequences at both 3' and 5' ends are required for splicing, this requirement does not extend past the 5' splice site sequence of the intron; and (iii) improving the 5' splice site of the internal exon to match the consensus sequence strongly decreases the accessibility of the upstream 3' splice site to antisense 2'-O-methyl oligoribonucleotides. This result most likely reflects changes in the strength of interactions near the 3' splice site in response to improvement of the 5' splice site and further supports the existence of communication between these sites across the exon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.