ArticleMolecular and cellular biology1994
Comparison of DNA methylation patterns among mouse cell lines by restriction landmark genomic scanning.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The CpG island in the murine foxl2 proximal promoter is differentially methylated in primary and immortalized cells.PloS one · 2013Article
- Maternal signals for progeny prevention against allergy and asthma.Cellular and molecular life sciences : CMLS · 2011Review
- Molecular marks for epigenetic identification of developmental and cancer stem cells.Clinical epigenetics · 2011Article
- Article
- Epigenetic regulation of human gamma-glutamyl hydrolase activity in acute lymphoblastic leukemia cells.American journal of human genetics · 2006Article
- High-throughput DNA methylation profiling using universal bead arrays.Genome research · 2006Article
- The study of aberrant methylation in cancer via restriction landmark genomic scanning.Oncogene · 2002Review
- Analyses of human gliomas by restriction landmark genomic scanning.Journal of neuro-oncology · 1997Article
- Establishment of methylation-sensitive-representational difference analysis and isolation of hypo- and hypermethylated genomic fragments in mouse liver tumors.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Altered DNA methylation and genome instability: a new pathway to cancer?Proceedings of the National Academy of Sciences of the United States of America · 1997Review
- Different patterns of DNA alterations detected by restriction landmark genomic scanning in heterogeneous prostate carcinomas.The American journal of pathology · 1997Article
- Restriction landmark cDNA scanning (RLCS): a novel cDNA display system using two-dimensional gel electrophoresis.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction landmark genomic scanning (RLGS) is a novel method which enables us to simultaneously visualize a large number of loci as two-dimensional gel spots. By this method, the status of DNA methylation can efficiently be determined by monitoring the appearance or disappearance of spots by using a methylation-sensitive restriction enzyme. In the present study, using RLGS with NotI, we examined, in comparison with a brain RLGS profile, the status of DNA methylation of more than 900 loci among three types of mouse cell lines: the embryonal carcinoma cell line P19, the stable mesenchymal cell line 10T1/2, and our established neuroepithelial (EM) cell lines. We found that the relative numbers of RLGS spots which appeared were less than 3.3% of those surveyed in all cell lines examined. However, 5 to 14% of spots disappeared, the numbers increasing with an increase in the length of the culture period, and many spots were commonly lost in 10T1/2 and in three EM cell lines. Thus, for these cell lines, many more spots disappeared than appeared. However, the numbers of spots disappearing and appearing were well balanced, and the ratio in P19 cells was almost equal to that in liver cells in vivo. These RLGS experimental observations suggested that permanent cell lines such as 10T1/2 are hypermethylated and that our newly established EM cell lines are also becoming heavily methylated at common loci. On the other hand, methylation and demethylation seem to be balanced in P19 cells in a manner similar to that in in vivo liver tissue.
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