ArticleMolecular and cellular biology1994
Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- DNA replication origins fire stochastically in fission yeast.Molecular biology of the cell · 2006Article
- Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication.Genes & development · 2005Article
- Coordination of replication and transcription along a Drosophila chromosome.Genes & development · 2004Article
- Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies.Molecular and cellular biology · 2002Article
- Multiple sites of replication initiation in the human beta-globin gene locus.Nucleic acids research · 2001Article
- Activity of the c-myc replicator at an ectopic chromosomal location.Molecular and cellular biology · 1999Article
- Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica.Molecular biology of the cell · 1999Article
- Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster.Molecular and cellular biology · 1999Article
- Transformation abrogates an early G1-phase arrest point required for specification of the Chinese hamster DHFR replication origin.The EMBO journal · 1998Article
- An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica.Molecular and cellular biology · 1997Article
- Identification of a predominant replication origin in fission yeast.Nucleic acids research · 1997Article
- Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts.The Journal of cell biology · 1996Article
- Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.Molecular and cellular biology · 1996Article
- Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae.Molecular biology of the cell · 1996Article
- Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels.Chromosoma · 1995Article
- Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure.Molecular and cellular biology · 1995Article
- The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites.Molecular and cellular biology · 1995Article
- Modular structural elements in the replication origin region of Tetrahymena rDNA.Nucleic acids research · 1995Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have mapped an initiation region of DNA replication at a single-copy chromosomal locus in exponentially proliferating Drosophila tissue culture cells, using two-dimensional (2D) gel replicon mapping methods and PCR-mediated analysis of nascent strands. The initiation region was first localized downstream of the DNA polymerase alpha gene by determining direction of replication forks with the neutral/alkaline 2D gel method. Distribution of replication origins in the initiation region was further analyzed by using two types of 2D gel methods (neutral/neutral and neutral/alkaline) and PCR-mediated nascent-strand analysis. Results obtained by three independent methods were essentially consistent with each other and indicated that multiple replication origins are distributed in a broad zone of approximately 10 kb. The nucleotide sequence of an approximately 20-kb region that encompasses the initiation region was determined and searched for sequence elements potentially related to function of replication origins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.