ArticleMolecular and cellular biology1994
The basic helix-loop-helix protein upstream stimulating factor regulates the cardiac ventricular myosin light-chain 2 gene via independent cis regulatory elements.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 78 citations in OpenAlex.
- Enhancing atrial-specific gene expression using a calsequestrin cis-regulatory module 4 with a sarcolipin promoter.The journal of gene medicine · 2018Article
- Myocyte-specific M-CAT and MEF-1 elements regulate G-protein gamma 3 gene (gamma3) expression in cardiac myocytes.DNA and cell biology · 2008Article
- Microphthalmia transcription factor isoforms in mast cells and the heart.Molecular and cellular biology · 2007Article
- Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay.Methods in molecular biology (Clifton, N.J.) · 2007Article
- Cross-regulatory interaction between Stra13 and USF results in functional antagonism.Oncogene · 2001Article
- Modulation of MLC-2v gene expression by AP-1: complex regulatory role of Jun in cardiac myocytes.Molecular and cellular biochemistry · 2001Article
- Transcriptional regulation of mouse delta-opioid receptor gene.The Journal of biological chemistry · 1999Article
- Transcription enhancer factor 1 interacts with a basic helix-loop-helix zipper protein, Max, for positive regulation of cardiac alpha-myosin heavy-chain gene expression.Molecular and cellular biology · 1997Article
- Mouse USF1 gene cloning: comparative organization within the c-myc gene family.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Combinatorial control of muscle development by basic helix-loop-helix and MADS-box transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- E-box sites and a proximal regulatory region of the muscle creatine kinase gene differentially regulate expression in diverse skeletal muscles and cardiac muscle of transgenic mice.Molecular and cellular biology · 1996Article
- Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.The EMBO journal · 1996Article
- Analysis of muscle creatine kinase gene regulatory elements in skeletal and cardiac muscles of transgenic mice.Molecular and cellular biology · 1996Article
- Fast-muscle-specific DNA-protein interactions occurring in vivo at the human aldolase A M promoter are necessary for correct promoter activity in transgenic mice.Molecular and cellular biology · 1996Article
- Regulation of the murine alpha B-crystallin/small heat shock protein gene in cardiac muscle.Molecular and cellular biology · 1995Article
- Inhibition of a signaling pathway in cardiac muscle cells by active mitogen-activated protein kinase kinase.Molecular biology of the cell · 1995Article
- An upstream stimulatory factor (USF) binding motif is critical for rat preprotachykinin-A promoter activity in PC12 cells.The Biochemical journal · 1995Article
- EFIA/YB-1 is a component of cardiac HF-1A binding activity and positively regulates transcription of the myosin light-chain 2v gene.Molecular and cellular biology · 1995Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Previous studies have documented that 250 bp of the rat cardiac ventricular myosin light-chain 2 (MLC-2v) promoter is sufficient to confer cardiac muscle-specific expression on a luciferase reporter gene in both transgenic mice and primary cultured neonatal rat myocardial cells. Utilizing ligation-mediated PCR to perform in vivo dimethyl sulfate footprinting, the present study has identified protein-DNA interaction within the position from -176 to -165. This region, identified as MLE1, contains a core sequence, CACGTG, which conforms to the consensus E-box site and is identical to the upstream stimulating factor (USF)-binding site of the adenovirus major late promoter. Transient assays of luciferase reporter genes containing point mutations of the site demonstrate the importance of this cis regulatory element in the transcriptional activation of this cardiac muscle gene in ventricular muscle cells. The protein complex that occupies this site is capable of binding to HF-1a and PRE B sites which are known to be required for cardiac muscle-specific expression of rat MLC-2v and alpha-myosin heavy-chain genes, respectively. This study provides direct evidence that USF, a member of the basic helix-loop-helix leucine zipper family, binds to MLE1, HF-1a, and PRE B sites and suggests that it is a component of protein complexes that may coordinately control the expression of MLC-2v and alpha-myosin heavy-chain genes. The current study also provides evidence that USF can positively and negatively regulate the MLC-2v gene via independent cis regulatory elements.
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