ArticleMolecular and cellular biology1994
Cap-dependent and cap-independent translation by internal initiation of mRNAs in cell extracts prepared from Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers.
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Who cites it
132 citing papers in PubMed, 274 citations in OpenAlex.
- Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications.Synthetic and systems biotechnology · 2026Review
- Circularization and Ribosome Recycling: From Polysome Topology to Translational Control.International journal of molecular sciences · 2026Review
- A flexible, high-throughput system for studying live mRNA translation with HiBiT technology.Nucleic acids research · 2025Article
- Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR).Nature structural & molecular biology · 2025Review
- Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiation.Cell systems · 2022Article
- The flip-flop configuration of the PABP-dimer leads to switching of the translation function.Nucleic acids research · 2022Article
- An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts.Nature communications · 2021Article
- Luciferase-based reporter system for in vitro evaluation of elongation rate and processivity of ribosomes.Nucleic acids research · 2021Article
- An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics.Journal of visualized experiments : JoVE · 2020Article
- Establishment of 5'-3' interactions in mRNA independent of a continuous ribose-phosphate backbone.RNA (New York, N.Y.) · 2020Article
- Functional Cyclization of Eukaryotic mRNAs.International journal of molecular sciences · 2020Article
- Translation of yes-associated protein (YAP) was antagonized by its circular RNA via suppressing the assembly of the translation initiation machinery.Cell death and differentiation · 2019Article
- Control of Translation at the Initiation Phase During Glucose Starvation in Yeast.International journal of molecular sciences · 2019Review
- A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation.ACS synthetic biology · 2019Article
- The 3' Untranslated Region of a Plant Viral RNA Directs Efficient Cap-Independent Translation in Plant and Mammalian Systems.Pathogens (Basel, Switzerland) · 2019Article
- Cap-Independent mRNA Translation in Germ Cells.International journal of molecular sciences · 2019Review
- Screening for functional IRESes using α-complementation system of β-galactosidase inBiotechnology for biofuels · 2019Article
- Article
- Sbp1 modulates the translation of Pab1 mRNA in a poly(A)- and RGG-dependent manner.RNA (New York, N.Y.) · 2018Article
- Specific RNP capture with antisense LNA/DNA mixmers.RNA (New York, N.Y.) · 2017Article
72 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Translation extracts were prepared from various strains of Saccharomyces cerevisiae. The translation of mRNA molecules in these extracts were cooperatively enhanced by the presence of 5'-terminal cap structures and 3'-terminal poly(A) sequences. These cooperative effects could not be observed in other translation systems such as those prepared from rabbit reticulocytes, wheat germ, and human HeLa cells. Because the yeast translation system mimicked the effects of the cap structure and poly(A) tail on translational efficiency seen in vivo, this system was used to study cap-dependent and cap-independent translation of viral and cellular mRNA molecules. Both the 5' noncoding regions of hepatitis C virus and those of coxsackievirus B1 conferred cap-independent translation to a reporter coding region during translation in the yeast extracts; thus, the yeast translational apparatus is capable of initiating cap-independent translation. Although the translation of most yeast mRNAs was cap dependent, the unusually long 5' noncoding regions of mRNAs encoding cellular transcription factors TFIID and HAP4 were shown to mediate cap-independent translation in these extracts. Furthermore, both TFIID and HAP4 5' noncoding regions mediated translation of a second cistron when placed into the intercistronic spacer region of a dicistronic mRNA, indicating that these leader sequences can initiate translation by an internal ribosome binding mechanism in this in vitro translation system. This finding raises the possibility that an internal translation initiation mechanism exists in yeast cells for regulated translation of endogenous mRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.