ArticleMolecular and cellular biology1994
Differential specificity for binding of retinoblastoma binding protein 2 to RB, p107, and TATA-binding protein.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
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- The histone demethylase Kdm5 controls Hid-induced cell death inFrontiers in cell death · 2024Article
- Domain architecture and protein-protein interactions regulate KDM5A recruitment to the chromatin.Epigenetics · 2023Review
- Functions and Interactions of Mammalian KDM5 Demethylases.Frontiers in genetics · 2022Review
- Identifying novel SMYD3 interactors on the trail of cancer hallmarks.Computational and structural biotechnology journal · 2022Article
- Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Autochthonous tumors driven byProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The histone demethylase KDM5A is required for the repression of astrocytogenesis and regulated by the translational machinery in neural progenitor cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Dynamic site-specific recruitment of RBP2 by pocket protein p130 modulates H3K4 methylation on E2F-responsive promoters.Nucleic acids research · 2018Article
- APC/C and retinoblastoma interaction: cross-talk of retinoblastoma protein with the ubiquitin proteasome pathway.Bioscience reports · 2016Review
- RBP2 induces stem-like cancer cells by promoting EMT and is a prognostic marker for renal cell carcinoma.Experimental & molecular medicine · 2016Article
- Article
- Histone demethylase RBP2 is critical for breast cancer progression and metastasis.Cell reports · 2014Article
- Dinosolve: a protein disulfide bonding prediction server using context-based features to enhance prediction accuracy.BMC bioinformatics · 2013Article
- Article
- Porphyromonas gingivalis invades human trophoblasts and inhibits proliferation by inducing G1 arrest and apoptosis.Cellular microbiology · 2009Article
- Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation.Molecular cell · 2008Article
- A novel protein with similarities to Rb binding protein 2 compensates for loss of Chk1 function and affects histone modification in fission yeast.Molecular and cellular biology · 2004Article
- Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Genetic alterations of the retinoblastoma-related gene RB2/p130 identify different pathogenetic mechanisms in and among Burkitt's lymphoma subtypes.The American journal of pathology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The growth suppressor activities of the RB and p107 products are believed to be mediated by the reversible binding of a heterogeneous family of cellular proteins to a conserved T/E1A pocket domain that is present within both proteins. To study the functional role of these interactions, we examined the properties of cellular retinoblastoma binding protein 2 (RBP2) binding to RB, p107, and the related TATA-binding protein (TBP) product. We observed that although RBP2 bound exclusively to the T/E1A pocket of p107, it could interact with RB through independent T/E1A and non-T/E1A domains and with TBP only through the non-T/E1A domain. Consistent with this observation, we found that a mutation within the Leu-X-Cys-X-Glu motif of RBP2 resulted in loss of ability to precipitate p107, while RB- and TBP-binding activities were retained. We located the non-T/E1A binding site of RBP2 on a 15-kDa fragment that is independent from the Leu-X-Cys-X-Glu motif and encodes binding activity for RB and TBP but does not interact with p107. Despite the presence of a non-T/E1A binding site, however, recombinant RBP2 retained the ability to preferentially precipitate active hypophosphorylated RB from whole-cell lysates. In addition, we found that cotransfection of RBP2 can reverse in vivo RB-mediated suppression of E2F activity. These findings confirm the differential binding specificities of the related RB, p107, and TBP proteins and support the presence of multifunctional domains on the nuclear RBP2 product which may allow complex interactions with the cellular transcription machinery.
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