ArticleMolecular and cellular biology1994
Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Regulatory differences between atypical and typical MAP kinases in Dictyostelium discoideum.Cellular signalling · 2025Article
- Atypical MAP kinases - new insights and directions from amoeba.Journal of cell science · 2023Review
- An atypical MAPK regulates translocation of a GATA transcription factor in response to chemoattractant stimulation.Journal of cell science · 2022Article
- MAPK docking motif in the Dictyostelium Gα2 subunit is required for aggregation and transcription factor translocation.Cellular signalling · 2021Article
- Mitogen-activated protein kinase regulation of the phosphodiesterase RegA in earlyMicrobiology (Reading, England) · 2020Article
- Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins inCells · 2019Article
- Extracellular polyphosphate signals through Ras and Akt to primeJournal of cell science · 2017Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Host signal transduction and protein kinases implicated in Legionella infection.Current topics in microbiology and immunology · 2013Review
- MAPKs in development: insights from Dictyostelium signaling pathways.Biomolecular concepts · 2011Article
- Tyrosine phosphorylation-mediated signaling pathways in dictyostelium.Journal of signal transduction · 2011Article
- Control of host cell phosphorylation by legionella pneumophila.Frontiers in microbiology · 2011Article
- MAP kinases have different functions in Dictyostelium G protein-mediated signaling.Cellular signalling · 2010Article
- A new Dictyostelium prestalk cell sub-type.Developmental biology · 2010Article
- G{alpha}5 subunit-mediated signalling requires a D-motif and the MAPK ERK1 in Dictyostelium.Microbiology (Reading, England) · 2010Article
- The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium.Developmental biology · 2009Article
- A Mitogen-activated protein kinase controls differentiation of bloodstream forms of Trypanosoma brucei.Eukaryotic cell · 2006Article
- Interacting protein kinases involved in the regulation of flagellar length.Molecular biology of the cell · 2006Article
- LmxMPK4, a mitogen-activated protein (MAP) kinase homologue essential for promastigotes and amastigotes of Leishmania mexicana.Kinetoplastid biology and disease · 2005Article
- Stage-specific requirement of a mitogen-activated protein kinase by Trypanosoma brucei.Molecular biology of the cell · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have cloned a developmentally regulated mitogen-activated protein kinase (extracellular signal-regulated kinase) from Dictyostelium discoideum designated ERK1. Using anti-pTyr antibodies, we show that ERK1 is phosphorylated on tyrosine in vivo and that it will phosphorylate myelin basic protein. The gene expresses two transcripts, one that is preferentially expressed during vegetative growth and early development and one that is induced during the multicellular stages. Developmental Western blots (immunoblots) using anti-ERK1 antibodies indicate that ERK1 is present throughout development. ERK1/lacZ reporter constructs suggest that, in the multicellular stages, the gene is preferentially expressed in a subpopulation of cells scattered throughout the organism, similar to the pattern seen with anterior-like cell markers. Antisense mutagenesis from a derepressible promoter indicates that ERK1 is essential for vegetative growth. Overexpression of ERK1 from either the Actin 15 promoter or the ERK1 promoter results in abnormal morphogenesis starting at the slug stage. Overexpression of ERK1 in null mutants of the phosphotyrosine phosphatase PTP2 results in the production of large aggregation streams and subsequent abnormal morphogenesis that indicate a genetic interaction between ERK1 and PTP2. These cells produce very large aggregation streams that break up into very small mounds that undergo abnormal morphogenesis. The genetic interaction between ERK1 and PTP2 appears to be specific since overexpression of ERK1 in a ptp1- null mutant does not produce the same phenotype. Our results indicate that ERK1 plays an essential role during the growth and differentiation of D. discoideum.
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