ArticleMolecular and cellular biology1994
Fast-muscle-specific expression of human aldolase A transgenes.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 38 citations in OpenAlex.
- DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Six1 and Eya1 expression can reprogram adult muscle from the slow-twitch phenotype into the fast-twitch phenotype.Molecular and cellular biology · 2004Article
- Expression of myogenin during embryogenesis is controlled by Six/sine oculis homeoproteins through a conserved MEF3 binding site.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Genomic sequences of aldolase C (Zebrin II) direct lacZ expression exclusively in non-neuronal cells of transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The human pH aldolase A promoter directs widespread but muscle-predominant expression in transgenic mice.Transgenic research · 1998Article
- DNA binding and transcriptional activation by the Ski oncoprotein mediated by interaction with NFI.Nucleic acids research · 1997Article
- A combination of MEF3 and NFI proteins activates transcription in a subset of fast-twitch muscles.Molecular and cellular biology · 1997Article
- Mouse USF1 gene cloning: comparative organization within the c-myc gene family.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- E-box sites and a proximal regulatory region of the muscle creatine kinase gene differentially regulate expression in diverse skeletal muscles and cardiac muscle of transgenic mice.Molecular and cellular biology · 1996Article
- Distinct regulatory elements control muscle-specific, fiber-type-selective, and axially graded expression of a myosin light-chain gene in transgenic mice.Molecular and cellular biology · 1996Article
- Common core sequences are found in skeletal muscle slow- and fast-fiber-type-specific regulatory elements.Molecular and cellular biology · 1996Article
- Independence and interdependence of the three human aldolase A promoters in transgenic mice.Gene expression · 1996Article
- Fast-muscle-specific DNA-protein interactions occurring in vivo at the human aldolase A M promoter are necessary for correct promoter activity in transgenic mice.Molecular and cellular biology · 1996Article
- Delineation of a slow-twitch-myofiber-specific transcriptional element by using in vivo somatic gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the human aldolase A gene is controlled by three alternative promoters. In transgenic mice, pN and pH are active in all tissues whereas pM is activated specifically in adult muscles composed mainly of fast, glycolytic fibers. To detect potential regulatory regions involved in the fast-muscle-specific activation of pM, we analyzed DNase I hypersensitivity in a 4.3-kbp fragment from the 5' end of the human aldolase A gene. Five hypersensitive sites were located near the transcription initiation site of each promoter in those transgenic-mouse tissues in which the corresponding promoter was active. Only one muscle-specific hypersensitive site was detected, mapping near pM. To functionally delimit the elements required for muscle-specific activity of pM, we performed a deletion analysis of the aldolase A 5' region in transgenic mice. Our results show that a 280-bp fragment containing 235 bp of pM proximal upstream sequences together with the noncoding M exon is sufficient for tissue-specific expression of pM. When a putative MEF-2-binding site residing in this proximal pM region is mutated, pM is still active and no change in its tissue specificity is detected. Furthermore, we observed a modulation of pM activity by elements lying further upstream and downstream from pM. Interestingly, pM was expressed in a tissue-specific way in all transgenic mice in which the 280-bp region was present (32 lines and six founder animals). This observation led us to suggest that the proximal pM region contains elements that are able to override to some extent the effects of the surrounding chromatin.
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