ArticleMolecular and cellular biology1994
The Ras/Raf signaling pathway is required for progression of mouse embryos through the two-cell stage.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 27 citations in OpenAlex.
- Reprogramming barriers in bovine cells nuclear transfer revealed by single-cell RNA-seq analysis.Journal of cellular and molecular medicine · 2022Article
- RASSF1A Regulates Spindle Organization by Modulating Tubulin Acetylation via SIRT2 and HDAC6 in Mouse Oocytes.Frontiers in cell and developmental biology · 2020Article
- Transcriptional Regulation of the First Cell Fate Decision.Journal of developmental biology & regenerative medicine · 2017Article
- Kras Is Critical for B Cell Lymphopoiesis.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Complementation of defective colony-stimulating factor 1 receptor signaling and mitogenesis by Raf and v-Src.Molecular and cellular biology · 1999Article
- K-ras is an essential gene in the mouse with partial functional overlap with N-ras.Genes & development · 1997Article
- Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have used microinjection of antisense oligonucleotides, monoclonal antibody, and the dominant negative Ras N-17 mutant to interfere with Ras expression and function in mouse oocytes and early embryos. Microinjection of either ras antisense oligonucleotides or anti-Ras monoclonal antibody Y13-259 did not affect normal progression of oocytes through meiosis and arrest at metaphase II. However, microinjection of fertilized eggs with constructs expressing Ras N-17 inhibited subsequent development through the two-cell stage. The inhibitory effect of Ras N-17 was overcome by simultaneous injection of a plasmid expressing an active raf oncogene, indicating that it resulted from interference with the Ras/Raf signaling pathway. In contrast to the inhibition of two-cell embryo development resulting from microinjection of pronuclear stage eggs, microinjection of late two-cell embryos with Ras N-17 expression constructs did not affect subsequent cleavages and development to morulae and blastocysts. It thus appears that the Ras/Raf signaling pathway, presumably activated by autocrine growth factor stimulation, is specifically required at the two-cell stage, which is the time of transition between maternal and embryonic gene expression in mouse embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.