ArticleMolecular and cellular biology1994
Tissue transglutaminase and apoptosis: sense and antisense transfection studies with human neuroblastoma cells.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 254 citations in OpenAlex.
- Biological Implications and Functional Significance of Transglutaminase Type 2 in Nervous System Tumors.Cells · 2024Review
- Microglial transglutaminase 2 deficiency causes impaired synaptic remodelling and cognitive deficits in mice.Cell proliferation · 2023Article
- GATA6-AS1 Regulates Intestinal Epithelial Mitochondrial Functions, and its Reduced Expression is Linked to Intestinal Inflammation and Less Favourable Disease Course in Ulcerative Colitis.Journal of Crohn's & colitis · 2023Article
- TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastoma.Acta neuropathologica communications · 2023Article
- Article
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- A composite of urinary biomarkers for differentiating between tubulointerstitial inflammation and interstitial fibrosis/tubular atrophy in kidney allografts.Annals of hepato-biliary-pancreatic surgery · 2018Article
- Development of new scaffolds as reversible tissue transglutaminase inhibitors, with improved potency or resistance to glutathione addition.MedChemComm · 2017Article
- Cyclin A1 expression and paclitaxel resistance in human ovarian cancer cells.European journal of cancer (Oxford, England : 1990) · 2016Article
- Transglutaminase 2 has opposing roles in the regulation of cellular functions as well as cell growth and death.Cell death & disease · 2016Review
- Prostate transglutaminase (TGase-4, TGaseP) enhances the adhesion of prostate cancer cells to extracellular matrix, the potential role of TGase-core domain.Journal of translational medicine · 2013Article
- ACTL6a enforces the epidermal progenitor state by suppressing SWI/SNF-dependent induction of KLF4.Cell stem cell · 2013Article
- Transglutaminase 2 contributes to apoptosis induction in Jurkat T cells by modulating Ca2+ homeostasis via cross-linking RAP1GDS1.PloS one · 2013Article
- Screening of therapeutic strategies for Huntington's disease in YAC128 transgenic mice.CNS neuroscience & therapeutics · 2012Review
- Presence of tissue transglutaminase in granular endoplasmic reticulum is characteristic of melanized neurons in Parkinson's disease brain.Brain pathology (Zurich, Switzerland) · 2011Article
- Extracellular transglutaminase 2 has a role in cell adhesion, whereas intracellular transglutaminase 2 is involved in regulation of endothelial cell proliferation and apoptosis.Cell proliferation · 2011Article
- Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminase.Malaria journal · 2011Article
- Type 2 transglutaminase in Huntington's disease: a double-edged sword with clinical potential.Journal of internal medicine · 2010Review
- Protein crosslinking by transglutaminase controls cuticle morphogenesis in Drosophila.PloS one · 2010Article
- Apoptotic factors (Bcl-2 and Bax) and diabetic retinopathy in type 2 diabetes.Journal of molecular histology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In this report, we show that the overexpression of tissue transglutaminase (tTG) in the human neuroblastoma cell line SK-N-BE(2) renders these neural crest-derived cells highly susceptible to death by apoptosis. Cells transfected with a full-length tTG cDNA, under the control of a constitutive promoter, show a drastic reduction in proliferative capacity paralleled by a large increase in cell death rate. The dying tTG-transfected cells exhibit both cytoplasmic and nuclear changes characteristic of cells undergoing apoptosis. The tTG-transfected cells express high Bcl-2 protein levels as well as phenotypic neural cell adhesion molecule markers (NCAM and neurofilaments) of cells differentiating along the neuronal pathway. In keeping with these findings, transfection of neuroblastoma cells with an expression vector containing segments of the human tTG cDNA in antisense orientation resulted in a pronounced decrease of both spontaneous and retinoic acid (RA)-induced apoptosis. We also present evidence that (i) the apoptotic program of these neuroectodermal cells is strictly regulated by RA and (ii) cell death by apoptosis in the human neuroblastoma SK-N-BE(2) cells preferentially occurs in the substrate-adherent phenotype. For the first time, we report here a direct effect of tTG in the phenotypic maturation toward apoptosis. These results indicate that the tTG-dependent irreversible cross-linking of intracellular protein represents an important biochemical event in the induction of the structural changes featuring cells dying by apoptosis.
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