ArticleJournal of virology1994
A small yeast RNA selectively inhibits internal initiation of translation programmed by poliovirus RNA: specific interaction with cellular proteins that bind to the viral 5'-untranslated region.
Article in Journal of virology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development.Animals : an open access journal from MDPI · 2022Article
- A La autoantigen homologue is required for the internal ribosome entry site mediated translation of giardiavirus.PloS one · 2011Article
- A peptide from autoantigen La blocks poliovirus and hepatitis C virus cap-independent translation and reveals a single tyrosine critical for La RNA binding and translation stimulation.Journal of virology · 2004Article
- Inhibitor RNA blocks the protein translation mediated by hepatitis C virus internal ribosome entry site in vivo.World journal of gastroenterology · 2004Article
- La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA.Nucleic acids research · 2002Article
- Novel fluorescence-based screen to identify small synthetic internal ribosome entry site elements.Molecular and cellular biology · 2001Article
- The human immunodeficiency virus type 1 gag gene encodes an internal ribosome entry site.Journal of virology · 2001Article
- Translational control of viral gene expression in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2000Review
- Posttranscriptional control of gene expression in yeast.Microbiology and molecular biology reviews : MMBR · 1998Review
- Nuclear import and the evolution of a multifunctional RNA-binding protein.The Journal of cell biology · 1998Article
- A small yeast RNA blocks hepatitis C virus internal ribosome entry site (HCV IRES)-mediated translation and inhibits replication of a chimeric poliovirus under translational control of the HCV IRES element.Journal of virology · 1998Article
- Sequences within a small yeast RNA required for inhibition of internal initiation of translation: interaction with La and other cellular proteins influences its inhibitory activity.Journal of virology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have purified, sequenced, and prepared a synthetic clone of a small (60-nucleotide) RNA molecule from the yeast Saccharomyces cerevisiae that had previously been isolated on the basis of its ability to selectively block the translation of poliovirus mRNA. RNA derived from the clone by transcription with T7 RNA polymerase appears to block translation initiation by internal ribosome entry (cap independent) but does not significantly affect cap-dependent translation. Deletion analysis of the poliovirus 5'-untranslated region (5'-UTR) has shown that yeast inhibitor RNA (I-RNA) requires internal ribosome entry site sequences to inhibit the translation of poliovirus RNA in vitro. Using a bicistronic RNA construct, we show that I-RNA preferentially inhibits translation by internal ribosome entry. Gel retardation and UV cross-linking studies demonstrate that I-RNA specifically binds proteins which interact with RNA secondary structures within the poliovirus 5'-UTR presumably involved in internal initiation. Specifically, purified I-RNA competes with virus RNA structures within the 5'-UTR which bind a cellular protein with an approximate molecular mass of 52 kDa. Finally, when transfected into HeLa cells, I-RNA efficiently inhibits the replication of poliovirus RNA presumably by inhibiting translation of the input virus RNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.