ArticleThe Journal of membrane biology1994
Polyamines block Ca(2+)-activated K+ channels in pituitary tumor cells (GH3).
Article in The Journal of membrane biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Targets of polyamine dysregulation in major depression and suicide: Activity-dependent feedback, excitability, and neurotransmission.Neuroscience and biobehavioral reviews · 2016Review
- Elevated polyamines in urothelial cells from OAB subjects mediate oxotremorine-evoked rapid intracellular calcium rise and delayed acetylcholine release.American journal of physiology. Renal physiology · 2013Article
- S100 calcium binding proteins and ion channels.Frontiers in pharmacology · 2012Article
- Hydrogen sulfide increases calcium-activated potassium (BK) channel activity of rat pituitary tumor cells.Pflugers Archiv : European journal of physiology · 2010Article
- Augmented bladder urothelial polyamine signaling and block of BK channel in the pathophysiology of overactive bladder syndrome.American journal of physiology. Cell physiology · 2009Article
- Roles of K+ channels in regulating tumour cell proliferation and apoptosis.Pflugers Archiv : European journal of physiology · 2004Review
- The effect of polyamines on KATP channels in guinea-pig ventricular myocytes.The Journal of physiology · 1998Article
- External action of di- and polyamines on maxi calcium-activated potassium channels: an electrophysiological and molecular modeling study.Biophysical journal · 1998Article
- Interactions of polyamines with ion channels.The Biochemical journal · 1997Review
- Rectification of rabbit cardiac ryanodine receptor current by endogenous polyamines.Biophysical journal · 1996Article
- Cadaverine induces closing of E. coli porins.The EMBO journal · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of the natural polyamines, putrescine, spermidine and spermine on single calcium-activated potassium channels from clonal rat pituitary tumor cells (GH3) were studied. Applied to inside-out patches, polyamines were found to reduce the current amplitude and open probability of the channels in a dose- and voltage-dependent manner, indicating that polyamines act as fast blockers which sense a fraction of the electrical field in the channel pore. The Kd for spermine was 11.2 mM for the reduction of unitary current amplitude and 0.7 mM for the reduction of the open probability. The order of effectiveness was spermine > spermidine > putrescine. From fitting beta-functions to current amplitude histograms, blocking and unblocking rates were determined as 11.4 x 10(4) sec-1 and 21.9 x 10(4) sec-1, respectively. The reduction of the channel open probability was relieved by an increase of the Ca2+ concentration of the internal solution, indicating that polyamines compete with Ca2+ at the Ca2+ sensor of the channel. Putrescine antagonized the effect of spermine on the channel current amplitude. The results suggest that polyamines at intracellular millimolar concentrations suppress ion channel activity and therefore may effect electrical discharge behavior of excitable cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.