ArticleJournal of clinical microbiology1994
General primer-mediated PCR for detection of Aspergillus species.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 2 syntheses or guidelines pooled it, 196 citations in OpenAlex.
- Diagnostic accuracy of PCR alone compared to galactomannan in bronchoalveolar lavage fluid for diagnosis of invasive pulmonary aspergillosis: a systematic review.Journal of clinical microbiology · 2012Pooled it
- Pooled it
- Aspergillus foetidus as a potent producer for β-galactosidase utilizing lemon peels and coffee waste powder: production optimization, purification, kinetic and thermodynamic characterization.Microbial cell factories · 2024Article
- Phenotypic Array for Identification and Screening of Antifungals againstJournal of fungi (Basel, Switzerland) · 2023Article
- Diagnosis of Superficial Mycoses by a Rapid and Effective PCR Method from Samples of Scales, Nails and Hair.Mycopathologia · 2018Article
- A risk assessment study of the occurrence and distribution of aflatoxigenicToxicology reports · 2018Article
- Rodent Models of Invasive Aspergillosis due toFrontiers in microbiology · 2017Review
- Diversity, molecular phylogeny and fingerprint profiles of airborne Aspergillus species using random amplified polymorphic DNA.World journal of microbiology & biotechnology · 2016Article
- A broader role for AmyR in Aspergillus niger: regulation of the utilisation of D-glucose or D-galactose containing oligo- and polysaccharides.Applied microbiology and biotechnology · 2012Article
- Spatial and developmental differentiation of mannitol dehydrogenase and mannitol-1-phosphate dehydrogenase in Aspergillus niger.Eukaryotic cell · 2010Article
- In vitro release by Aspergillus fumigatus of galactofuranose antigens, 1,3-beta-D-glucan, and DNA, surrogate markers used for diagnosis of invasive aspergillosis.Journal of clinical microbiology · 2006Article
- Aspergillus niger mstA encodes a high-affinity sugar/H+ symporter which is regulated in response to extracellular pH.The Biochemical journal · 2004Article
- Semiquantitative detection by real-time PCR of Aspergillus fumigatus in bronchoalveolar lavage fluids and tissue biopsy specimens from patients with invasive aspergillosis.Journal of clinical microbiology · 2003Article
- Mannitol is required for stress tolerance in Aspergillus niger conidiospores.Eukaryotic cell · 2003Article
- Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis.Journal of clinical microbiology · 2003Article
- ABC transporters of the wheat pathogen Mycosphaerella graminicola function as protectants against biotic and xenobiotic toxic compounds.Molecular genetics and genomics : MGG · 2003Article
- Nested PCR assays for detection of Blastomyces dermatitidis DNA in paraffin-embedded canine tissue.Journal of clinical microbiology · 2003Article
- ABC transporters and azole susceptibility in laboratory strains of the wheat pathogen Mycosphaerella graminicola.Antimicrobial agents and chemotherapy · 2002Article
- Current status of nonculture methods for diagnosis of invasive fungal infections.Clinical microbiology reviews · 2002Review
- The Aspergillus niger faeB gene encodes a second feruloyl esterase involved in pectin and xylan degradation and is specifically induced in the presence of aromatic compounds.The Biochemical journal · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR assay was developed for the diagnosis of invasive aspergillosis in immunocompromised patients. For this purpose, the complete nucleotide sequences of the genes encoding the 18S rRNA of Aspergillus nidulans, Aspergillus terreus, Aspergillus niger, and Aspergillus flavus were elucidated and aligned to the sequences of Aspergillus fumigatus and other clinically relevant prokaryotic and eukaryotic microorganisms. Genus-specific sequences could be identified in the V7 to V9 region of 18S rRNA. By using hot-start PCR, Southern blot hybridization, and restriction enzyme analysis, Aspergillus-specific and -sensitive determination was achieved. Five of six immunosuppressed mice experimentally infected with A. fumigatus developed infection, and rRNA could be detected in each case, even in livers with the absence of positive cultures. Aspergillus species were detected by PCR in four neutropenic patients with proven aspergillosis, although Aspergillus species had been isolated from only one bronchoalveolar lavage (BAL) fluid sample. Aspergillus species were detected by PCR in two more patients suspected of having infection. Positive PCR signals were obtained from the BAL samples of 3 of 8 neutropenic patients who had developed pulmonary infiltrates, but none were obtained from the samples of 14 nonimmunosuppressed patients. These results indicate the potential value of PCR to detect Aspergillus species in BAL samples and, therefore, to identify neutropenic patients at risk for invasive aspergillosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.